Imac ni2 sepharose

The 4-nitrophenyl-α-d-galactopyranoside (pNP-α-Gal), d-galactose (Gal), and 4-nitrophenyl-α-N-acetylgalactosaminide (pNP-α-NAcGal) were purchased from Sigma-Aldrich Chemical Company (St. Louis, MO, USA). Encyclo DNA-polymerase and enterokinase were purchased from Evrogen JSC (16/10 Miklukho-Maklaya str., Moscow, Russian Federation). Nco I and Sal I were purchased from New England Biolabs (NEB), Ipswich, MA, USA. pET 40 b(+) plasmid was purchased from Invitrogen, Carlsbad, CA, USA. Bacto-tryptone, sorbitol, MgCl₂, KH₂PO₄, kanamycin, glycerin phenyl methanesulfonyl fluoride PMSF were purchased from (Helicon, Moscow, Russian Federation, Kutuzovsky Prospect, 88). Sodium phosphates one- and two-substituted were purchased from PanReac AppliChem GmbH (Ottoweg 4, Darmstadt, Germany). IMAC Ni²⁺ Sepharose, Q-Sepharose, Mono-Q, and Superdex-200 PG were purchased from GE Healthcare (Uppsala, Sweden). EtOH, trifluoroacetic acid (TFA), CD₃OD were from the Russian Federation.

The 4-nitrophenyl-α-d-galactopyranoside (pNP-α-Gal), melibiose (Gal-α-(1→6)-Glc), galactose (Gal), glucose (Glc), Bovine serum albumin (BSA), NaN₃, and 2,5-dihydroxybenzoic acid were purchased from Sigma Chemical Company (St. Louis, MO, USA). Encyclo DNA-polymerase and enterokinase were purchased from Evrogen (Moscow, Russian Federation). Sodium phosphates, one- and two-substituted, were purchased from PanReac AppliChem GmbH (Darmstadt, Germany). IMAC Ni²⁺ Sepharose, Q-Sepharose, Mono-Q, and Superdex-200 PG were purchased from GE Healthcare (Uppsala, Sweden). Heavy-oxygen water was purchased from Component Reactive (Moscow, Russia).