Reverse tablet molds were designed in FreeCAD and 3D-printed with a MakerBot Ultimaker 3. PDMS molds were prepared using SYLGARD 184 kits (Sigma, cat #761028). Peptide solutions were prepared at 2 mM in 1X PBS, incubated for 3–4 h at room temperature to fibrillize, and mixed with sugars to a final concentration of 0.67 mM peptide and 7.8 wt% each of trehalose (Santa Cruz Biotechnology, cat #394303), 20,000 Da dextran (Alfa Aesar, cat# J61216), and mannitol (Sigma, cat #M4125). Adjuvanted formulations contained cholera toxin B (List Biological, cat #104) or Vaccigrade cyclic-di-AMP (Invivogen, cat #vac-nacda) at doses indicated in figure captions. Final solutions were pipetted into the PDMS tray (30 μL per tablet), frozen at −80 °C, and lyophilized. Heating was performed by placing individual tablets in microcentrifuge tubes in a heating block set at 45 °C. KLH groups were heated as solutions in their final formulation.
Trehalose
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Trehalose is a disaccharide sugar that is commonly used in various laboratory applications. It functions as a cryoprotectant, stabilizing proteins and biomolecules during freezing and drying processes. Trehalose helps maintain the structural integrity and activity of sensitive biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Sylgard 184 kits, by Merck Group (2 mentions)
Mannitol, by Merck Group (2 mentions)
Ribose, by Merck Group (1 mentions)
Xylose, by Merck Group (1 mentions)
Mannose, by Merck Group (1 mentions)
Arabinose, by Merck Group (1 mentions)
Galactose, by Merck Group (1 mentions)
Myo inositol, by Merck Group (1 mentions)
Hexamethyldisilazane hmds, by Merck Group (1 mentions)
Phenyl β d glucopyranoside, by Merck Group (1 mentions)
Ultra pure, by Thermo Fisher Scientific (1 mentions)
Cellobiose, by Merck Group (1 mentions)
Sucrose, by Merck Group (1 mentions)
Trifluoroacetic acid tfa, by Merck Group (1 mentions)
Pyridine, by Merck Group (1 mentions)
Fructose, by Merck Group (1 mentions)
Lactose, by Merck Group (1 mentions)
Raffinose, by Merck Group (1 mentions)
Hydroxylamine hydrochloride, by Merck Group (1 mentions)
3 protocols using trehalose
1
Lyophilized Peptide Tablets with Adjuvants
2
Carbohydrate Standard Protocol for GC-MS
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Glucose, fructose, sucrose, myo-inositol, mannose, xylose, ribose, lactose, arabinose, and galactose were purchased from Sigma-Aldrich (St. Louis, MO, USA). Galactinol, ribitol, and trehalose were supplied by Santa Cruz Biotechnology (Santa Cruz, CA, USA). Raffinose and cellobiose were obtained from Fluka (Sigma-Aldrich, St. Louis, MO, USA) and Supelco (Sigma-Aldrich, St. Louis, MO, USA), respectively. Stachyose was from TCI America (Tokyo, Japan). All standard reagents were of analytical grade. Standard sugar solutions were prepared by dissolving individual sugars in ultra-pure (liquid chromatography mass spectrometry-grade, 18 MΩ) water (Fisher Scientific, Pittsburgh, PA, USA). The concentration of stock solution for each sugar component was either 1 or 10 mg/mL. Chemical reagents for derivatization prior to GC–MS analyses, including pyridine, phenyl-β-D -glucopyranoside, hydroxylamine hydrochloride, hexamethyldisilazane (HMDS) and trifluoroacetic acid (TFA) were purchased from Sigma-Aldrich (St. Louis, MO, USA).
3
Freeze-dried Nanofiber Tablet Fabrication
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Reverse tablet molds were designed in FreeCAD and three-dimensionally printed with MakerBot Ultimaker 3. Polydimethylsiloxane (PDMS) molds were prepared using SYLGARD 184 kits (Sigma-Aldrich, 761028). Fibrillized nanofiber solutions were mixed with adjuvants and sugars to a final concentration of 7.8 weight % each of trehalose (Santa Cruz Biotechnology, 394303), dextran (Alfa Aesar, J61216), and mannitol (MilliporeSigma, M4125). Final solutions were pipetted into the PDMS tray (30 μl per tablet), frozen at −80°C, and lyophilized (46 ).
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