The lentiviral hMAGEA2 shRNA vectors (shMAGEA2 sequence: 5′-GATAATCGTCCTGGCC ATAAT-3′) for the knockdown of hMAGEA2 were purchased from Sigma. Oligonucleotides were cloned into the pLKO.1 lentiviral vector. HEK293T cells were co-transfected with pLKO.1-scramble or pLKO.1-shMAGEA2 and pMDLg/p RRE, pMD2.G, and pRSV-Rev using FuGENE HD transfection reagent (Promega). The MDA-MB-468 cells were infected with lentiviruses encoding shRNA using 8 μg/mL protamine sulfate (Sigma). After 48 h, cells were selected by puromycin (1 μg/ml) for 4 days, establishing a stable knockdown of hMAGEA2 in the MDA-MB-468 cell line.
Lentiviral hmagea2 shrna vectors
Manufactured by Merck Group
Lentiviral hMAGEA2 shRNA vectors are a type of laboratory equipment used in genetic research. They are designed to deliver short hairpin RNA (shRNA) targeting the human MAGEA2 gene into cells using a lentiviral delivery system. The primary function of these vectors is to enable the knockdown or silencing of the MAGEA2 gene expression in experimental settings, allowing researchers to study the effects of MAGEA2 gene modulation.
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2 protocols using lentiviral hmagea2 shrna vectors
1
Lentiviral-mediated hMAGEA2 Knockdown
2
Lentiviral Knockdown of hMAGEA2 in Melanoma Cells
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The lentiviral hMAGEA2 shRNA vectors (sh-hMAGEA2 sequence: 5′-GATAATCGTCCTGGCCATAAT-3′) for the knockdown of hMAGEA2 were purchased from Sigma. Oligonucleotides were cloned into the pLKO.1 lentiviral vector. HEK293T cells were co-transfected with pLKO.1-scramble or pLKO.1-shMAGEA2 and pMDLg/p RRE, pMD2.G, and pRSV-Rev using FuGENE HD transfection reagent (Promega). SK-MEL-5 and SK-MEL-28 cells were infected with lentiviruses encoding shRNA using 8 µg/ml protamine sulfate (Sigma). After 48 h, cells were selected by puromycin (1 µg/ml) for 4 days to establish a stable knockdown of hMAGEA2 in the SK-MEL-5 and SK-MEL-28 cell lines.
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