Determiner gl e kit

The blood glucose levels, serum insulin levels, triglyceride (TG) content in the liver, and glycogen content in the liver were determined using a Glutest Neo Super (Sanwa Chemical Co., Tokyo, Japan), an insulin kit (Morinaga, Tokyo, Japan), a leptin kit (Morinaga) and the Determiner-L TG II kit and the Determiner-GL-E kit (Wako Pure Chemical Industries, Osaka, Japan), respectively. The plasma alanine aminotransferase, free fatty acids, total cholesterol, LDL-cholesterol, and triglyceride levels were assayed using enzymatic methods (Wako Pure Chemical Industries). The plasma 3-hydroxybutyrate level was determined using the β Hydroxybutyrate Assay kit (Abcam, Cambridge, England). The blood glucose levels were checked just before and 4 hours after the administration of OSI-906 or the vehicle until day 7 or on days 8, 10, 14, 21, and 28. Blood samples were taken from the inferior vena cava immediately after sacrifice.

The plasma glucose levels were determined using Glutest Neo Super (Sanwa Chemical Co. Kanagawa, Japan) just before and 4 h after the administration of OSI-906 or the vehicle. Serum insulin levels were determined using an insulin kit (Morinaga Institute of Biological Science, Yokohama, Japan). The glycogen content in the liver was determined using a Determiner-GL-E Kit (Wako Pure Chemical Industries, Osaka, Japan). The serum glutamic pyruvic transaminase (GPT), free fatty acid (FFA), and triglyceride (TG) levels were assayed using enzymatic methods (Wako Pure Chemical Industries, Osaka, Japan).