Harmaline (4,9-dihydro-7-methoxy-1-methyl-3H-pyrido[3,4-b] indole), harmine (7-methoxy-1-methyl-9H-pyrido[3,4-b] indole), harmane methosulfate (1-methyl-9H-pyrido[3,4-b] indole, methosulfate), norharmanium methiodide (2-methyl-9H-β-carboline-2-ium, iodide), 2,9-dimethyl-4,9-dihydro-3Hβ-carbolin-2-ium, iodide], and MTT (thiazolyl blue tetrazolium, bromide) were obtained from Sigma-Aldrich (St. Louis, MO). [3H]MPP+(85Ci/mmol) was purchased from American Radiolabelled Chemicals (St. Louis, MO). ASP+(4-(4-(diethylamino) styryl)-N-methylpyridinium, iodide) and trypan blue were purchased from Life Technologies, Inc. (Carlsbad, CA). Cell culture media and reagents were from Life Technologies, Inc. LC-MS/MS grade acetonitrile, water, and formic acid were purchased from Fisher Scientific (Waltham, MA).
Cell culture media and reagents
Manufactured by Thermo Fisher Scientific
Sourced in United States
Cell culture media and reagents are essential laboratory products used to support the growth, maintenance, and study of cells in controlled in vitro environments. These products provide the necessary nutrients, growth factors, and other components to sustain cell lines and primary cells for various research and diagnostic applications.
Automatically generated - may contain errors
Lab products found in correlation
Celltiter blue reagent, by Promega (3 mentions)
Formic acid, by Thermo Fisher Scientific (2 mentions)
Cisplatin, by Merck Group (2 mentions)
Protease inhibitor cocktail, by Merck Group (2 mentions)
Ab41621, by Abcam (2 mentions)
Histone h4 sc 25260, by Santa Cruz Biotechnology (2 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (2 mentions)
Reaction ready first strand cdna synthesis kit, by Qiagen (2 mentions)
Tri reagent, by Merck Group (2 mentions)
Mda mb 231, by American Type Culture Collection (2 mentions)
Nuclease protease free water, by Quality Biological (2 mentions)
Bca protein assay kit, by Thermo Fisher Scientific (2 mentions)
Trypan blue, by Thermo Fisher Scientific (1 mentions)
Harmaline, by Merck Group (1 mentions)
Lc ms ms grade acetonitrile, by Thermo Fisher Scientific (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions)
Harmine, by Merck Group (1 mentions)
Doxorubicin, by Merck Group (1 mentions)
Cpt 11, by Merck Group (1 mentions)
47 protocols using cell culture media and reagents
1
Evaluation of Monoamine Uptake Inhibitors
2
Evaluating Cell Death Pathways
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell culture media and reagents were obtained from Life Technologies (Grand Island, NY, USA). CPT-11, doxorubicin, etoposide and thapsigargin were from Sigma-Aldrich (St.Louis, MO, USA). 3- methyladenine (3-MA) was from MP Biomedicals, LLC (Solon, OH, USA), MG132, necrostatin-1 (NEC-1) were from EMD Biosciences (San Diego, CA, USA), U0126 was from Promega Corporation (Madison, WI, USA).
3
Cellular Metabolite Extraction Protocol
4
Protease Inhibitors and Lipid Synthesis Inhibitors
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Complete protease inhibitor cocktail tablets were obtained from Roche (Laval, QC, Canada). [3H]Acetic acid was from PerkinElmer (Waltham, MA, USA). Cell culture media and reagents were from Life Technologies (Burlington, ON, Canada). TOFA (5‐(tetradecyloxy)‐2‐furoic acid) and C75 ((2R*,3S*)‐tetrahydro‐4‐methylene‐2‐octyl‐5‐oxo‐3‐furancarboxylic acid) were purchased from Cayman Chemical (Ann Arbor, MI, USA). All other chemicals were purchased from Sigma (St. Louis, MO, USA).
5
Quantification of 2-Hydroxyglutarate
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All liquid chromatography solvents and additives were obtained from Sigma-Aldrich (St. Louis, MO), as well as all chemicals needed for the synthesis of 2HG. Stable isotope-labeled alpha-ketoglutarate was purchased from Cambridge Isotope Laboratories (Tewksbury, MA). Cell culture media and reagents were purchased from Life Technologies (Grand Island, NY), and IDH1 (R132H/+) cells were purchased from Horizon Discovery Ltd. (Cambridge, UK). An enzyme-linked immunosorbent assay (ELISA) kit for measuring D-2HG dehydrogenase was purchased from Cloud Clone Corp. (Houston, TX).
6
Estradiol and Tamoxifen Peptide Study
7
Glutamate Transporter Regulation Assay
8
Cytotoxicity Assay with Cisplatin
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cisplatin, β-actin antibody and thiazolyl blue tetrazolium bromide were purchased from Sigma (St Louis, Missouri, USA). Antibodies against eIF3a, XPC and p27kip1 were from Santa Cruz Biotechnology (Santa Cruz, California, USA). Cell culture media and reagents were obtained from Invitrogen (Carlsbad, California, USA). All other reagents were of molecular biology grade.
9
MDA-MB 231 Breast Cancer Cell Viability Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
MDA-MB 231 breast
cancer cells were purchased from ATCC (Manassas, VA). Cell culture
media and reagents were purchased from Invitrogen. 3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium
(MTS)-cell viability assay reagents were purchased from Promega. Cells
were cultured in high glucose DMEM containing 10% fetal bovine serum.
Cells were plated in 96-well microtiter plates at 5000 cells per well
and incubated for 2 h to allow for adherence. Where appropriate, pH
7.4 media was exchanged for pH 6.0 media adjusted with 1 M HCl to
determine PA cytotoxicity at acidic pH. After 2 h, 10 μL of
PA was added for the final appropriate concentrations. Cell viability
was measured after 48 h using an MTS cell viability assay and was
used according to the supplier’s instructions. Briefly, the
cell media was replaced after 48 h with a stock of 20% MTS solution
in pH 7.4 media. The plate was incubated for 1–3 h, and absorbance
was read using a Molecular Devices microplate reader (490 nm). Cell
viability was calculated as an absorbance percent relative to the
untreated cell control at the same pH. Experiments were performed
in triplicate.
cancer cells were purchased from ATCC (Manassas, VA). Cell culture
media and reagents were purchased from Invitrogen. 3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium
(MTS)-cell viability assay reagents were purchased from Promega. Cells
were cultured in high glucose DMEM containing 10% fetal bovine serum.
Cells were plated in 96-well microtiter plates at 5000 cells per well
and incubated for 2 h to allow for adherence. Where appropriate, pH
7.4 media was exchanged for pH 6.0 media adjusted with 1 M HCl to
determine PA cytotoxicity at acidic pH. After 2 h, 10 μL of
PA was added for the final appropriate concentrations. Cell viability
was measured after 48 h using an MTS cell viability assay and was
used according to the supplier’s instructions. Briefly, the
cell media was replaced after 48 h with a stock of 20% MTS solution
in pH 7.4 media. The plate was incubated for 1–3 h, and absorbance
was read using a Molecular Devices microplate reader (490 nm). Cell
viability was calculated as an absorbance percent relative to the
untreated cell control at the same pH. Experiments were performed
in triplicate.
10
Antioxidant Mechanisms in Cell Culture
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!