Nb100 2466

Manufactured by Novus Biologicals

Sourced in United States

The NB100-2466 is a laboratory equipment product manufactured by Novus Biologicals. It is designed for general laboratory use, but a detailed factual description of its core function cannot be provided without the risk of extrapolation or interpretation.

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Lab products found in correlation

Alexa fluor 488, by Abcam (1 mentions) Ab32678, by Abcam (1 mentions) Sc 11418, by Santa Cruz Biotechnology (1 mentions)

2 protocols using nb100 2466

Lrmp-ITPR2 Interaction in Intestinal Tuft Cells

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The direct interaction of Lrmp and ITPR2 proteins in the small intestine was detected in situ by Duolink^TM secondary antibodies and a detection kit (Sigma) according to the manufacturer’s instructions. FFPE intestinal tissue sections were subjected to the Lrmp and ITPR2 proximal assays as described in the published paper³⁴. In brief, tissue sections were incubated with anti-Lrmp (Biorbyt, orb166443, 1:50 dilution) and anti-ITPR2 (Novus, NB100-2466, 1:50 dilution) antibodies together with Duolink secondary antibodies. Ligation step was performed to ligate the secondary antibodies that are in close proximity. Anti-Dclk1 Alexa Fluor® 488 (Abcam, ab202754, 1:100 dilution) antibodies were used for IF staining on the same tissue slide to detect the tuft cells. DAPI was used for counterstaining the nucleus.

Chang C.Y., Wang J., Zhao Y., Liu J., Yang X., Yue X., Wang H., Zhou F., Inclan-Rico J.M., Ponessa J.J., Xie P., Zhang L., Siracusa M.C., Feng Z, & Hu W. (2021). Tumor suppressor p53 regulates intestinal type 2 immunity. Nature Communications, 12, 3371.

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Immunocytochemical Analysis of Subcellular Proteins

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Immunocytochemistry was performed as previously described^{11 (link)} using the following
antibodies: mouse monoclonal anti-nuclear pore complex proteins antibody
(ab60080, Abcam, Cambridge, UK), mouse monoclonal anti-SERCA2a and mouse
monoclonal anti-RyR antibody (MA3-919 and MA3-916, Thermo Scientific, Rockford,
IL, USA), goat polyclonal anti-IP₃R2 antibody (NB100-2466, Novus
Biologicals, Littleton, CO, USA), rabbit polyclonal anti-P-CaMKII antibody
(ab32678, Abcam, Cambridge, UK) and rabbit polyclonal anti-HDAC4 antibody
(sc-11418, Santa Cruz Biotechnology, Santa Cruz, CA, USA). The specificity of
the antibodies was confirmed in Western blots.

Ljubojevic S., Radulovic S., Leitinger G., Sedej S., Sacherer M., Holzer M., Winkler C., Pritz E., Mittler T., Schmidt A., Sereinigg M., Wakula P., Zissimopoulos S., Bisping E., Post H., Marsche G., Bossuyt J., Bers D.M., Kockskämper J, & Pieske B. (2014). Early Remodelling of Perinuclear Ca2+ Stores and Nucleoplasmic Ca2+ Signalling During the Development of Hypertrophy and Heart Failure. Circulation, 130(3), 244-255.

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