Tcrvα2 b20

Manufactured by Thermo Fisher Scientific

TCRVα2 (B20.1) is a monoclonal antibody that specifically binds to the alpha chain of the T cell receptor (TCR). It is a useful tool for the identification and isolation of T cells expressing the TCRVα2 chain.

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Lab products found in correlation

Streptavidin pe cy7, by Thermo Fisher Scientific (2 mentions) Streptavidin apc cy7, by BioLegend (1 mentions) Streptavidin percp cy5, by Thermo Fisher Scientific (1 mentions) Facsaria 3, by BD (1 mentions) Lsrfortessa x 20, by BD (1 mentions) Lsrfortessa, by BD (1 mentions) Intracellular fixation and permeabilization buffer set, by Thermo Fisher Scientific (1 mentions)

2 protocols using tcrvα2 b20

Multicolor Flow Cytometry Antibodies

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Most fluorochrome-conjugated antibodies, unless otherwise indicated, were purchased from Biolegend (San Diego, CA) or eBioscience (San Diego, CA), including: CD3 (17A2), CD8 (53–6.7), CD11b (M1/70), CD11c (N418), CD16 and CD32/Fc block (93), CD19 (1D3), CD45R/B220 (RA3-6B2), CD115/CSF-1R (AFS98), Gr-1 (RB6-8C5), Ly-6C (HK1.4), MHC-II (M5/114.15.2), NK1.1 (PK136), Ter 119 (TER-119), TCRVα2 (B20.1), and TCRVβ5.1 (MR9-4). Streptavidin–PE-Cy7, streptavidin–PerCP–Cy5.5, and 7-amino-actinomycin D (7-AAD) viability staining solutions were obtained from eBioscience, and streptavidin-APC-Cy7 was obtained from Biolegend. ExtrAvidin−TRITC was obtained from Sigma-Aldrich (St. Louis, MO).

Yang Y.W, & Luo W.H. (2017). Recruitment of bone marrow CD11b+Gr-1+ cells by polymeric nanoparticles for antigen cross-presentation. Scientific Reports, 7, 44691.

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Flow Cytometry for Immune Cell Analysis

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All cells were preincubated with CD16/32 Fc blocking antibody before staining related antibodies to eliminate nonspecific binding of antibodies to FcγR. Flow cytometry was performed on a LSRFortessa (BD) or LSRFortessaTMX‐20 (BD), and cell sorting was done on a FACSAriaTM III (BD). The flow data were analysed with FlowJo (Three Star). In this study, the monoclonal antibodies of CD2 (RM2‐5), CD3 (145‐2C11), CD4 (GK1.5), CD8a (53‐6.7), CD19 (eBio1D3), CD31 (390), CD41 (eBioMWReg30), CD45 (30‐F11), c‐Kit (2B8), CD201 (eBio 1560), CD11b (M1/70), B220 (RA3‐6B2), Gr1 (RB6‐8C5), NK1.1 (RK136), CD11c (N418), Ter119 (TER‐119), Sca‐1 (D7) CD44 (IM7), CD62L (MEL‐14), CD69 (H1.2F3), CD25 (PC61.5) TCR Vα2 (B20.1), TCR Vβ5 (MR9‐4), and Streptavidin‐PE‐Cy7 were brought from eBioscience or BioLegend. Rag2^−/−‐OT1‐iR9‐PSCs were stained intracellularly with eBioscience Intracellular Fixation and Permeabilization Buffer Set.

Wu B., Zhang Q., Hong P., Liu L., Peng H., Xia C., Wang T., Wang Y., Weng Q., Liu X., Geng Y., Wang J, & Wu H. (2023). Antigen‐specific TCR‐T cells from Rag2 gene‐deleted pluripotent stem cells impede solid tumour growth in a mouse model. Cell Proliferation, 56(4), e13389.

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