Superoxide dismutase sod kit

A total of 50 Wistar male rats weighing 220.51±10.24 g were selected and purchased from Shanghai Slaccas Experimental Animal Co., Ltd., with a production license of SCXK (Shanghai) 2012-0002, fed at a constant temperature of 22°C with normal circadian rhythm and free diet. Iva was purchased from Servier Laboratories. Trimetazidine was purchased from Beijing Wansheng Pharmaceutical Co., Ltd. Sodium pentobarbital was purchased from Hubei Hongyun Long Biological Technology Co. Ltd. Connective tissue growth factor (CTGF) polyclonal antibody was purchased from Invitrogen; Thermo Fisher Scientific, Inc. Superoxide dismutase (SOD) kit was purchased from Dojindo Molecular Technologies, Inc. Primary mouse anti-rat CTGF (cat. no. AMAB91366; dil, 1:1,000) and β-actin monoclonal antibodies (cat. no. A1978; dil, 1:400), secondary rabbit anti-mouse polyclonal antibody (cat. no. SAB3701212; dil, 1:100) used in western blot analysis were purchased from Sigma-Aldrich; Merck KGaA. BCA quantitative kit was purchased from Beijing Solarbio Science & Technology Co., Ltd. RT-qPCR kit and minScript reverse transcription kit were purchased from Takara Biotechnology Co., Ltd. Biological signal recorder system was purchased from Anhui Zhenghua Biological Instrument Equipment Co., Ltd.
The study was approved by the Ethics Committee of The Affiliated Hospital of Jining Medical University (Jining, China).