Glass capillaries were pulled as described (Dandekar et al., 2013 ). Needles were backfilled with a solution containing 1 μM fMLP (F3506; Sigma-Aldrich) and 100 nM Alexa 430 succinimidyl ester (A-10169; Invitrogen, Carlsbad, CA) and held by a micromanipulator (MM-89; Narishige, East Meadow, NY). Agonist flow rate from the pipette was controlled as described (Dandekar et al., 2013 ). Cells were labeled with CellMask Orange (Life Technologies, Carlsbad, CA) according to manufacturer's instructions.
Fmlp f3506
Manufactured by Merck Group
Sourced in United States
FMLP (F3506) is a synthetic peptide used in cell biology research. It functions as a chemoattractant, stimulating the migration of certain types of immune cells. This product is intended for research use only and its core function is to induce cell chemotaxis.
Automatically generated - may contain errors
Lab products found in correlation
Cellmask orange, by Thermo Fisher Scientific (1 mentions)
Mm 89, by Narishige (1 mentions)
Triton x 100, by Merck Group (1 mentions)
Dmi8 microscope, by Leica (1 mentions)
Giemsa, by Merck Group (1 mentions)
Fibronectin, by Merck Group (1 mentions)
D glucose g7021, by Merck Group (1 mentions)
M1902, by Merck Group (1 mentions)
Polydimethylsiloxane pdms, by Dow (1 mentions)
Neutrophil isolation kit, by STEMCELL (1 mentions)
2 protocols using fmlp f3506
1
Directed Cell Chemotaxis Assay
2
Neutrophil Isolation and FITC-Dextran Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Neutrophil isolation kit (17957, STEMCELL, Vancouver, Canada); Bovine serum albumin (A1933-25G, Sigma-Aldrich, St. Louis, MO, USA), FBS (iCell-0500, iCell Bioscience Inc, Shanghai, China); RPMI 1640 (SH30809.01, Hyclone, Logan, UT, USA); Fluorescein (FITC-dextran, 10 kDa; final concentration 5 μM, Sigma-Aldrich, St. Louis, MO, USA); fMLP (F3506, 1 mM, Sigma-Aldrich, St. Louis, MO, USA); Triton X-100 (9036-19-5, Sigma, St. Louis, MO, MO, USA); Giemsa (G5637, Sigma, St. Louis, MO, USA); D-glucose (G7021, Sigma-Aldrich, St. Louis, MO, USA); mannitol (10 mM, an osmotic control reagent, M1902, Sigma-Aldrich, St. Louis, MO, USA); Fibronectin (F2006, 2 μg mL−1, Sigma-Aldrich, St. Louis, MO, USA); Commercial magnetic Neutrophil isolation kit (17957, STEMCELL Technologies, Inc., Toronto, Canada); Precursors and initiators of Polydimethylsiloxane (PDMS) (Dow Corning, Midland, MI, USA); Glass slide (80350-0001, Jiangsu Shitai Xinchuang Scientific Instrument Co., Ltd., Nanjing, China); Microscope (DMi8, Leica, Wezler, Hesse, Germany); Plasma cleaner (PDC-002, Harrick Scientific Products, Inc., New York, NY, USA).
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