All herbal medicines were provided by Guangyuan Hospital of Traditional Chinese Medicine (Guangyuan, China). Fetal bovine serum (FBS) and Dulbecco’s modified Eagle medium (DMEM) were purchased from the Gibco Co. (Grand Island, NY, United States). BCA protein assay reagents, SDS-polyacrylamide gel electrophoresis (SDS-PAGE) preparation kit, penicillin-streptomycin mixture, trypsin, phosphate buffer saline (PBS) and cell counting kit-8 (CCK-8) were purchased from Boster Biological Technology Co., Ltd. (Wuhan, China). Annexin V-FITC/PI assay kit was purchased from Multisciences (Lianke) Biotechnology Corporate Limited (Hangzhou, China). H2O2 was purchased from Chengdu Chron Chemicals Co. Ltd. (Chengdu, China). Primary antibodies for PI3K, phosphorylation-PI3K (p-PI3K), AKT, phosphorylation-AKT (p-AKT), Nrf2, Bcl-2, Bax and cleaved-caspase-3 (C- caspase-3) were obtained from the ImmunoWay Biotechnology Co. (Suzhou, China). The assay kits for MDA, SOD, CAT, and GSH-PX were purchased from the Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Ultrapure water purified by Millipore Ultra-pure Water Purifier (Millipore, Milford, MA, United States) was used. Other reagents were all of analytical grade.
Annexin 5 fitc pi assay kit
Manufactured by MultiSciences Biotech
Sourced in China
The Annexin V-FITC/PI assay kit is a laboratory tool used to detect and quantify apoptotic cells. It utilizes the binding properties of Annexin V, a protein that has a high affinity for phosphatidylserine, which is externalized on the surface of apoptotic cells. The kit also includes propidium iodide (PI) to distinguish between early apoptotic and late apoptotic/necrotic cells.
Automatically generated - may contain errors
Lab products found in correlation
Bca protein assay reagent, by Boster Bio (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Cell counting kit 8 cck 8, by Boster Bio (1 mentions)
Phosphate buffer saline (pbs), by Boster Bio (1 mentions)
Dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (1 mentions)
Trypsin, by Boster Bio (1 mentions)
Assay kits for mda, by Nanjing Jiancheng (1 mentions)
Bcl 2, by Immunoway (1 mentions)
Bcl2 associated x (bax), by Immunoway (1 mentions)
Gsh px, by Nanjing Jiancheng (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
Jnk1 2, by Cell Signaling Technology (1 mentions)
P jnk1 2, by Cell Signaling Technology (1 mentions)
C caspase9, by Cell Signaling Technology (1 mentions)
C caspase 3, by Cell Signaling Technology (1 mentions)
Jc 1 probe, by US Everbright (1 mentions)
2 protocols using annexin 5 fitc pi assay kit
1
In Vitro Evaluation of Antioxidant and Antiapoptotic Effects of Traditional Chinese Medicine
2
Comprehensive Phytochemical Profile Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Reference substances including malic acid, citric acid, gallic acid, ephedrine, pseudoephedrine, neomangiferin, mangiferin, albiflorin, paeoniflorin, prim-O-glucosylcimifugin, liquiritin, 5-O-β-D-glucosyl-5-O-methylcisamminol, anemarsaponin E1, anemarsaponin BII, liquiritigenin, anemarsaponin C, licorice saponin G2, glycyrrhizic acid and anemarspaonin I were acquired from the Chengdu Desite Co. Ltd (Chengdu, China), and the purity of these compounds was ≥98%. Annexin V-FITC/PI assay kit was obtained from Multisciences (Lianke) Biotechnology Corporate Limited (Hangzhou, China). CCK8 detection kit and JC-1 probe was obtained from US Everbright®Inc (Suzhou, China). Enhanced chemiluminescence (ECL) luminescence reagent was purchased from Beijing 4A Biotech Co (Beijing, China). NF-κB activity assay kits were purchased from Beyotime Biotech (Haimen, China), and the human tumor necrosis factor (TNF)-α was obtained from Pepro Tech (Rocky Hill, NJ, China). BCA protein quantitative kit and primary antibodies for JAK2, STAT3, Bcl-2, Bax were bought from BOSTER biological technology company (Wuhan, China). C-caspase-9, C-caspase-3, p-JNK1/2, JNK1/2, β-actin, and the IgG-HRP were obtained from Cell Signaling technology (Chiyoda-ku, Tokyo, Japan). The QuickEasy™ Cell Direct RT-qPCR Kit-SYBR Green I kit was obtained from Chengdu Foregene Company Limited.
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