Chromium controller and chromium single cell 3 gem reagent kits v3

Gene expression libraries from were prepared from FACS-sorted populations of single cells using the Chromium Controller and Chromium Single Cell 3’ GEM Reagent Kits v3 (10x genomics, Inc.) according to the manufacturer’s protocol. The resulting sequencing libraries comprised of standard Illumina paired-end constructs flanked with P5 and P7 sequences. The 16 bp 10x barcode and 10 bp UMI were encoded in read 1, while read 2 was used to sequence the cDNA fragment. Sample index sequences were incorporated as the i7 index read. Paired-end sequencing (2 × 150 bp) was performed on the Illumina NovaSeq 6000 platform. The resulting.bcl sequence data were processed for QC purposes using bcl2fastq software (v2.20.0.422) and the resulting fastq files were assessed using FastQC (v0.11.3), FastqScreen (v0.9.2) and FastqStrand (v0.0.5) prior to alignment and processing with the CellRanger (v6.1.2) pipeline.

Gene expression libraries from were prepared from FACS-sorted populations of single cells using the Chromium Controller and Chromium Single Cell 3’ GEM Reagent Kits v3 (10x genomics, Inc.) according to the manufacturer’s protocol. The resulting sequencing libraries comprised of standard Illumina paired-end constructs flanked with P5 and P7 sequences. The 16 bp 10x barcode and 10 bp UMI were encoded in read 1, while read 2 was used to sequence the cDNA fragment. Sample index sequences were incorporated as the i7 index read. Paired-end sequencing (2 × 150 bp) was performed on the Illumina NovaSeq 6000 platform. The resulting.bcl sequence data were processed for QC purposes using bcl2fastq software (v2.20.0.422) and the resulting fastq files were assessed using FastQC (v0.11.3), FastqScreen (v0.9.2) and FastqStrand (v0.0.5) prior to alignment and processing with the CellRanger (v6.1.2) pipeline.