An Agilent 7890B GC system coupled with an Agilent 240 ion trap mass spectrometer was used, equipped with a 7693 auto-sampler and a G4513A injector. Data processing and analysis were performed by the MS workstation (version 7.0.2, Agilent technologies). GC-MS runs and data analysis were performed in the same manner as described before19 (link),25 (link). The 13 selected menthol-like substances and nicotine were quantified (by quantifier ion of analytical standard) in waterpipe samples where the respective flavoring was positively identified (by qualifier ion of analytical standard). Concentrations of the menthol-like substances are averages of duplicate extractions. Limits of detection (LOD) were calculated based on the calibration curve as S/N 3/1; the limit of quantification (LOQ) was set as the lowest point of the calibration curve (10 μg/mL).
240 ion trap mass spectrometer
Manufactured by Agilent Technologies
The 240 ion trap mass spectrometer is a laboratory instrument designed for the analysis of chemical samples. It utilizes an ion trap to capture and analyze ions, providing information about the composition and structure of the sample. The core function of this product is to perform precise and sensitive mass spectrometry measurements.
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Lab products found in correlation
4 protocols using 240 ion trap mass spectrometer
1
Quantification of Menthol-like Substances in Waterpipe Samples
2
Quantification of Flavourings and Nicotine in Waterpipe Samples
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An Agilent 7890B GC system coupled with an Agilent 240 ion trap mass spectrometer was used, equipped with a 7693 auto-sampler and a G4513A injector. GC–MS runs and data analysis were performed in the same manner as described before.24 (link)
The 11 selected flavourings and nicotine were quantified (by quantifier ion of analytical standard) in waterpipe samples where the respective flavouring was positively identified (by qualifier ion of analytical standard). Concentrations of the flavourings in the duplicate runs were averaged for further analyses. Results were reported as semiquantitative since no spike recovery experiments were performed. Limits of detection (LODs) were calculated based on the calibration curve as 3 * SD/slope; limits of quantification were set as the lowest and highest point of the calibration curve <10 and>100 µg/mL.
The 11 selected flavourings and nicotine were quantified (by quantifier ion of analytical standard) in waterpipe samples where the respective flavouring was positively identified (by qualifier ion of analytical standard). Concentrations of the flavourings in the duplicate runs were averaged for further analyses. Results were reported as semiquantitative since no spike recovery experiments were performed. Limits of detection (LODs) were calculated based on the calibration curve as 3 * SD/slope; limits of quantification were set as the lowest and highest point of the calibration curve <10 and>100 µg/mL.
3
GC-MS Analysis of Ormenis eriolepis Fractions
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The GC-MS analyses of Ormenis eriolepis Dichloromethane Fraction (Oe-DF) and Hexanic Extract (Oe-HE) were carried out at the Instrumental Technical Services of the “Estación Experimental del Zaidín” (CSIC, Granada, Spain). Briefly, 1 μl of the derivative solution was injected in a Varian 450GC coupled to 240 Ion Trap Mass Spectrometer as detector. The injection conditions were: splitless mode with 1 minute duration pulse, the injector temperature was 250°C; the He column flow was 1 ml/minute in a capillary column (Varian Factor Four VF-5 ms 30mx0.25mmx0.25 pm). For Mass spectrometry conditions, the EI ionization was 70 eV, the transfer line was at 280°C and the Trap at 240°C, mass range acquisition was from m/z 50 to m/z 500 and cared in Full Scan mode. Qualitative analysis of compounds was based on the comparison of their spectral mass and their relative Retention time with those of NIST08 mass spectra database and Kovats RI on the chromatograms recorded in Full Scan or in SIM mode usin g the characteristics ions. Quantitative analysis was realized by integration of peaks and calculated as percent of total identified area on the TIC chromatograms.
4
GC-MS Analysis of Retama monosperma Hexane Extract
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The GC-MS analyses of Retama monosperma hexanic extract (Rm-HE) were carried out at the Instrumental Technical Services of the “Estación Experimental del Zaidín” (CSIC, Granada, Spain). Briefly, 1 μl of the derivative solution was injected in a Varian 450GC coupled to 240 Ion Trap Mass Spectrometer as detector. The injection conditions were: splitless mode with 1 minute duration pulse, the injector temperature was 250°C; the He column flow was 1 ml/minute in a capillary column (Varian Factor Four VF-5 ms 30 m×0.25 mm×0.25 μm). For Mass spectrometry conditions, the EI ionization was 70 eV, the transfer line was at 280°C and the Trap at 240°C, mass range acquisition was from m/z 50 to m/z 500 and cared in Full Scan mode. Qualitative analysis of compounds was based on the comparison of their spectral mass and their relative Retention time with those of NIST08 mass spectra database and Kovats RI on the chromatograms recorded in Full Scan or in SIM mode using the characteristics ions. Quantitative analysis was realized by integration of peaks and calculated as percent of total identified area on the TIC chromatograms.
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