Gfp 1020

Manufactured by Santa Cruz Biotechnology

Sourced in United States

The GFP-1020 is a fluorescence microscopy imaging system designed for the detection and analysis of green fluorescent protein (GFP) in biological samples. The core function of the GFP-1020 is to provide a reliable and sensitive platform for visualizing and quantifying GFP-labeled cells, tissues, or proteins.

Automatically generated - may contain errors

Lab products found in correlation

Ab18465, by Takara Bio (1 mentions) A11122, by Abcam (1 mentions) Sc 991, by Santa Cruz Biotechnology (1 mentions) Alexa 568 goat antibody to rabbit igg, by Thermo Fisher Scientific (1 mentions) Alexa 647 goat antibody to rabbit igg, by Thermo Fisher Scientific (1 mentions) Alexa 488 goat antibody to rabbit igg, by Thermo Fisher Scientific (1 mentions) Alexa 488 goat antibody to mouse igg, by Thermo Fisher Scientific (1 mentions) Alexa 568 antibody to mouse igg, by Thermo Fisher Scientific (1 mentions) Prb 278p, by Fortrea (1 mentions) Nel744001kt, by PerkinElmer (1 mentions) Ab3786, by Santa Cruz Biotechnology (1 mentions) Freezing microtome, by Leica camera (1 mentions) Sc 346, by Santa Cruz Biotechnology (1 mentions) Alexa fluor 488 or 568 conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions) Dab reagent, by Vector Laboratories (1 mentions) Abc kit, by Vector Laboratories (1 mentions) Bx53f microscope, by Olympus (1 mentions) Alexa conjugated secondary antibodies, by Jackson ImmunoResearch (1 mentions) Af3075, by R&D Systems (1 mentions) Ab9610, by R&D Systems (1 mentions)

7 protocols using gfp 1020

Immunohistochemical Localization of Neural Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Single and double immunohistochemical reactions were performed as described previously (Garcia-Moreno et al., 2012 (link)) using the following primary antibodies: Rabbit antibody to EGFP (Molecular Probes, A11122, 1:1000), mouse antibody to EGFP (Abcam, ab1218, 1:1000), chick antibody to EGFP (Aves GFP 1020, 1:10000), rabbit anti Nurr1 (Santa Cruz, sc-991, 1:400), rabbit antibody to Dbx1 (1:200; kind gift by Prof. Nakagawa, Univ. Minessota, United States), rabbit antibody to Tbr1 (Chemicon, AB9616, 1:1,000), rat antibody to Ctip2 (Abcam, ab18465, 1:500), rabbit antibody to dsRed2 (Takara, 632475, 1:1000), and rabbit antibody to Pax6 (Covance, PRB-278P, 1:200). Dbx1 immunostaining required antigen retrieval with citrate acid.
For secondary antibodies (all 1:1000), we used Alexa 568 goat antibody to rabbit IgG (Molecular Probes, A11011), Alexa 647 goat antibody to rabbit IgG (Molecular Probes, A21245), Alexa 488 goat antibody to rabbit IgG (Molecular Probes, A11034), Alexa 488 goat antibody to mouse IgG (Molecular Probes, A11001), Alexa 568 antibody to mouse IgG (Molecular Probes, A11004), Alexa 568 goat antibody to rat IgG (Molecular Probes, A11077), and Alexa 488 goat antibody to chicken (Invitrogen, A11039).

Rueda-Alaña E., Martínez-Garay I., Encinas J.M., Molnár Z, & García-Moreno F. (2018). Dbx1-Derived Pyramidal Neurons Are Generated Locally in the Developing Murine Neocortex. Frontiers in Neuroscience, 12, 792.

+ Open protocol

+ Expand

Paraformaldehyde Lung Tissue Fixation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Lungs were inflated with 4% paraformaldehyde to 25 cm H₂O pressure for 10 min and then removed and submerged in 4% paraformaldehyde in PBS for 4 h at 4°C. For tissues used for phospho-Smad1/5/8 detection, PhosStop (Roche 4906845001) was added to the fixative. Tissue was dehydrated, embedded in paraffin and sectioned at 7 µm. Tissue sections underwent 10 mM sodium citrate antigen retrieval and were blocked with 3% BSA, 10% donkey serum and 0.1% Triton X-100 for 1 h at room temperature. Primary antibodies diluted in block were applied and incubated overnight at 4°C. Tissue sections were washed with PBS and fluorophore-conjugated secondary antibodies were diluted at 1:500 and incubated for 1 h at room temperature. For phospho-Smad1/5/8 staining, HRP-conjugated secondary antibody (1:1000) and TSA detection system were used (PerkinElmer, NEL744001KT). Primary antibodies were as follows: LAMP-3/CD208 (Dendritics, DDX0191, 1:200), endomucin (Santa Cruz, sc-65495, 1:250), GFP (Aves lab, GFP-1020, 1:500), HOPX (Santa Cruz, sc-398703, 1:50), RFP (Rockland, 600401379, 1:250), PDGFRβ (Cell Signaling, 3169, 1:100), RAGE/AGER (R&D, MAB1179, 1:200), SFTPC (Millipore, ab3786, 1:500; Santa Cruz, SC-7706, 1:100), phospho-Smad1/5/8 (Millipore, AB3848-I, 1:250; Cell Signaling, 9511). Images were obtained using Zeiss LSM 710, LSM 780 and Imager AxioCam microscopes.

Chung M.I., Bujnis M., Barkauskas C.E., Kobayashi Y, & Hogan B.L. (2018). Niche-mediated BMP/SMAD signaling regulates lung alveolar stem cell proliferation and differentiation. Development (Cambridge, England), 145(9), dev163014.

+ Open protocol

+ Expand

Immunohistochemical Detection of pSTAT3

Check if the same lab product or an alternative is used in the 5 most similar protocols

For the detection of pSTAT3, food was removed at the onset of the light cycle. Animals were treated four hours later with metreleptin (5 mg/kg, i.p.) and subjected to perfusion 90 min after treatment. These and all other mice for immunohistochemical analysis were anesthetized with a lethal dose of pentobarbital and transcardially perfused with phosphate buffered saline (PBS) followed by 10% buffered formalin. Brains were removed, placed in 10% buffered formalin overnight, and dehydrated in 30% sucrose for several days. Using a freezing microtome (Leica), brains were cut into 30 μm sections. Sections were treated sequentially with 1% hydrogen peroxide/0.5% sodium hydroxide, 0.3% glycine, 0.03% sodium dodecyl sulfate, and blocking solution (PBS with 0.1% triton, 3% Normal Donkey Serum). Immunostaining was performed using primary antibodies for pSTAT3 (Cell Signaling #9145, 1:1000), GFP (Aves Labs #GFP1020, 1:1000), STAT1 (Santa Cruz sc-346, 1:250). All antibodies were reacted with species-specific Alexa Fluor-488 or -568 conjugated secondary antibodies (Invitrogen, 1:200) or processed with the avidin-biotin/diaminobenzidine (DAB) method (ABC kit, Vector Labs, 1:500; DAB reagents, Sigma). Images were collected on an Olympus BX53F microscope. DAB images were pseudocolored using Photoshop software.

Pan W., Allison M.B., Sabatini P., Rupp A., Adams J., Patterson C., Jones J.C., Olson D.P, & Myers MG J.r. (2019). Transcriptional and physiological roles for STAT proteins in leptin action. Molecular Metabolism, 22, 121-131.

+ Open protocol

+ Expand

Immunofluorescent Labeling of Brain Sections

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mice were transcardially perfused with 4% paraformaldehyde. The brains were removed, post-fixed overnight, and cut into 50 μm coronal and sagittal sections using a vibratome (Leica Microsysteme, Rueil Malmaison, France). Immunofluorescent labeling was performed on sections or on cultured cells fixed with paraformaldehyde 4%. The following antibodies were used: anti-GFP (rabbit, 1/500; Life Technologies, A11122. Chicken, 1/500; Aves Labs GFP-1020), anti-DCX (goat, 1/100; Santa Cruz Sc-8066), anti-MBP (mouse IgG1, 1/500; Chemicon MAB384), anti-GFAP (mouse IgG 1/1,000; Sigma G3893), anti-OLIG2 (rabbit, 1/500; Chemicon AB 9610), anti-SOX9 (goat, 1/200; R&D AF3075), anti-PDGFRα (rat, 1/250; Chemicon CBL1366), anti-CC1 (mouse, 1/500; Calbiochem OP80), anti-CASPR/PARANODIN (rabbit, 1/500; L51, gift from Dr Goutebroze), anti-TCF4 (mouse IgG2a, 1/500; Millipore 05–511), anti-PSA-NCAM (mouse, ½; supernatant produced in our laboratory), anti-NEUROFILAMENT-160 (mouse, 1/500; Sigma N5264), and anti-βIV-SPECTRIN (mouse clone 393/2, 1/500; NeuroMab AB_2315816). The sections and cells were incubated with appropriate Alexa-conjugated secondary antibodies (1/500; Jackson ImmunoResearch Laboratories) then counterstained with Hoechst 33,342 (1/1,000; Sigma). Images were captured with a Zeiss apotome system (20× and 60× objectives) and a Zeiss 510 confocal (60× objective).

El Waly B., Cayre M, & Durbec P. (2018). Promoting Myelin Repair through In Vivo Neuroblast Reprogramming. Stem Cell Reports, 10(5), 1492-1504.

+ Open protocol

+ Expand

Pancreatic Endocrine Immunohistochemistry

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immunohistochemistry was performed according to standard procedures and analyzed with a Leica SP8 confocal microscope. The whole endocrine portion of the pancreas was scanned in all larvae analyzed. Confocal stacks were analyzed with ImageJ software. The contrast was adjusted in most images, and Figs 1G and 7A and B were processed with the “smooth” function. The following antibodies were used: anti‐GFP (1:250, Aves Labs GFP‐1020), anti‐Igfbp1 (1:50, Santa Cruz sc‐13097) anti‐dsRed (1:500, Clontech 632496), anti‐Insulin (1:200, Sigma I8510), anti‐Glucagon (1:200, Sigma G6254), and anti‐Pdx1 (gift from Chris Wright). β‐cell proliferation was assessed by adding 10 mM EdU to eggwater supplemented with 10 mM Hepes (Thermo), and measuring EdU incorporation with the Click‐iT EdU Alexa Fluor 647 imaging kit (Invitrogen), according to the manufacturer's protocol.

Lu J., Liu K., Schulz N., Karampelias C., Charbord J., Hilding A., Rautio L., Bertolino P., Östenson C., Brismar K, & Andersson O. (2016). IGFBP1 increases β‐cell regeneration by promoting α‐ to β‐cell transdifferentiation. The EMBO Journal, 35(18), 2026-2044.

+ Open protocol

+ Expand

Multimodal Immunolabeling Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Primary antibodies used in this study included: rabbit Iba1 (1:500, Wako, 019-19741), guinea pig Iba1 (1:500, Synaptic Systems, 234 004), mouse RV capsid (1:500, Abcam, ab34749), rat Sox2 (1:500, Invitrogen, 14-9811-82), chicken GFP (1:1000, Aves labs, GFP-1020), mouse NOVA1 (1:500, Santa Cruz, sc100334), rabbit EOMES (1:200, Sigma-Aldrich, HPA028896), chicken NeuN (1:1,000, Millipore, ABN91), rabbit IFITM3 (1:500, Proteintech, 11714-1-AP).

Popova G., Retallack H., Kim C.N., Wang A., Shin D., DeRisi J.L, & Nowakowski T. (2023). Rubella virus tropism and single-cell responses in human primary tissue and microglia-containing organoids. eLife, 12, RP87696.

+ Open protocol

+ Expand

Immunofluorescent Characterization of Kidney

Check if the same lab product or an alternative is used in the 5 most similar protocols

Kidneys were fixed in 4% paraformaldehyde in phosphate-buffered saline (PBS) for 15 minutes, incubated overnight in 10% sucrose/PBS at 4°C, and imbedded in OCT (Fisher Scientific). Cryosections (8–9μm) were incubated overnight with primary antibodies in 5% heat-inactivated sheep serum/PBST (PBS with 0.1% Triton X-100). We used primary antibodies for GFP (1:500, Aves GFP-1020), Wt1 (1:100, Santa Cruz sc-7385), Cytokeratin (1:200, Sigma C2562), and Six2 (1:200, Abcam ab277942). Fluorophore-labeled secondary antibodies were used for indirect visualization of the target. Images were taken with a Nikon Ti-2 inverted widefield microscope equipped with an Andor Zyla camera and Lumencor SpectraX light source housed at the Confocal Imaging Core (CIC) at Cincinnati Children’s Hospital Medical Center (CCHMC).

Chung E., Deacon P., Hu Y.C., Lim H.W, & Park J.S. (2023). Hedgehog signaling is required for the maintenance of mesenchymal nephron progenitors. bioRxiv.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!