Anti ubiquitin fl 76

Manufactured by Santa Cruz Biotechnology

Anti-ubiquitin (FL-76) is a primary antibody product offered by Santa Cruz Biotechnology. This antibody recognizes the ubiquitin protein, which is a small regulatory protein found in most tissues of eukaryotic organisms. The core function of this antibody is to detect and bind to ubiquitin, which is commonly used in research applications involving the study of ubiquitin-related cellular processes.

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Lab products found in correlation

Bovine serum albumin (bsa), by New England Biolabs (1 mentions) Duolink in situ proximity ligation assay system, by Olink (1 mentions) Anti myc ab9106, by Abcam (1 mentions) Anti sumo1 c9h1, by Cell Signaling Technology (1 mentions) Chloroquine, by Merck Group (1 mentions) Anti tubulin dm1α, by Merck Group (1 mentions) Anti smn, by Cell Signaling Technology (1 mentions) Lc3 l7543, by Merck Group (1 mentions) Anti β actin ac 74, by Merck Group (1 mentions) Mg132, by Merck Group (1 mentions) Anti p53, by Santa Cruz Biotechnology (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Cycloheximide (chx), by Merck Group (1 mentions)

Close spelling variants of this product

Ubiquitin (134 citations) Anti-ubiquitin (129 citations)

3 protocols using anti ubiquitin fl 76

Ubiquitin Deubiquitination Assay Protocol

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Reactions were prepared with 10μl protein-G beads from an Ede1 immuno-isolation, 10ng purified DUB, buffer to final concentrations of: 50mM Tris pH 7.5, 1mM dithiothreitol, 100μg/ml BSA (NEB) and rotated at room temperature for 60 minutes. Reactions were stopped by addition of 10μl of 8M urea (final concentration 1.25M) and 12.5μl of 5X SDS sample buffer (final concentration 1X). Samples were analyzed by SDS-PAGE and immuno blotting with anti-Ede1 (Linda Hicke) and anti-ubiquitin (FL-76 Santa Cruz Biotech) sera.

Weinberg J.S, & Drubin D.G. (2014). Regulation of clathrin-mediated endocytosis by dynamic ubiquitination and deubiquitination. Current biology : CB, 24(9), 951-959.

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Visualizing Protein Interactions in Cell Lines

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HeLa or HuT-102 cells were fixed in methanol onto glass coverslips or by cytospin, respectively. Protein-protein interactions were visualized using the Duolink in situ proximity ligation assay (PLA) system (Olink Bioscience), employing anti-Tax, anti-Urm1 [32 (link)], anti-SUMO-1 C9H1 (Cell Signaling Technology), anti-ubiquitin FL-76 (Santa Cruz) and anti-Myc ab9106 (Abcam).

Hleihel R., Khoshnood B., Dacklin I., Omran H., Mouawad C., Dassouki Z., El-Sabban M., Shirinian M., Grabbe C, & Bazarbachi A. (2018). The HTLV-1 oncoprotein Tax is modified by the ubiquitin related modifier 1 (Urm1). Retrovirology, 15, 33.

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Protein Modulation and Detection Assay

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MG-132, cycloheximide, chloroquine, and DMSO were purchased from Sigma-Aldrich. Only chloroquine was solubilized in Milli-Q water. The following antibodies were used: anti-SMN (1:2000, 2F1, Cell Signaling Technologies); anti-p53 (PAb1801, Ref); p21 (F-5, Santa Cruz); anti-ubiquitin (FL-76, Santa Cruz); p62 (P0067, Sigma-Aldrich); LC3 (L7543, Sigma-Aldrich); anti-tubulin (DM1α, 1:4000, Sigma-Aldrich); and anti-β-actin (AC-74, 1:4000, Sigma-Aldrich).

Rietz A., Li H., Quist K.M., Cherry J.J., Lorson C.L., Burnett B., Kern N.L., Calder A.N., Fritsche M., Lusic H., Boaler P.J., Choi S., Xing X., Glicksman M.A., Cuny G.D., Androphy E.J, & Hodgetts K.J. (2017). Discovery of a Small Molecule Probe That Post-Translationally Stabilizes the Survival Motor Neuron Protein for the Treatment of Spinal Muscular Atrophy. Journal of medicinal chemistry, 60(11), 4594-4610.

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