Images of the GMS particles processed by the PGSS technique were obtained by scanning electron microscopy (SEM, EVO LS 15, Zeiss, Oberkochen, Germany) to evaluate the morphology, size, homogeneity, and surface of the particles. Prior to imaging, SLMPs were sputtered-coated (Q150T S/E/ES, Quorum Technologies, Lewes, UK) with a layer of 10 nm of iridium to improve the contrast. Particle sizes were measured by image analysis using ImageJ v1.49 software (National Institutes of Health, Bethesda, MD, USA).
Q150t s e es
Manufactured by Quorum Technologies
Sourced in United Kingdom
The Q150T S/E/ES is a sputter coater designed for the deposition of thin conductive or protective films onto samples for microscopy and microanalysis applications. It features a compact and user-friendly design with automatic control of the sputter process parameters.
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Lab products found in correlation
4 protocols using q150t s e es
1
Morphological Analysis of GMS Particles
2
Microparticle Morphology Visualization
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Microparticle morphology was visualised by field emission scanning electron microscopy (FESEM Ultra Plus, Zeiss, Jena, Germany). Briefly, the dry powder was placed onto metal plates and 5-nm thick iridium film was sputter-coated (model Q150T S/E/ES, Quorum Technologies, Lewes, UK) on the sample before visualisation.
3
Morphological Characterization of KGM Microparticles
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The size and shape of unloaded KGM microparticles and the three formulations of KGM/INH/RFB microparticles were studied using Morphologi 4 ® (Malvern Instruments Limited, Malvern, UK). A small amount of dry powder was placed on the device and dispersed on a glass plate by compressed air. The dispersed particles were analysed by a high-resolution microscope with a broad size range between 0.5 and 50 µm. The geometric diameter, circularity, elongation and convexity were calculated as the mean of 15 000 particles for each formulation.
Morphological characterisation of drug-loaded KGM microparticles was also performed by field emission scanning electron microscopy (FESEM Ultra Plus, Zeiss, Jena, Germany). Dry powders were placed onto metal plates and 5 nm thick iridium film was sputter-coated (model Q150T S/E/ES, Quorum Technologies, Lewes, UK) on the samples before viewing.
Morphological characterisation of drug-loaded KGM microparticles was also performed by field emission scanning electron microscopy (FESEM Ultra Plus, Zeiss, Jena, Germany). Dry powders were placed onto metal plates and 5 nm thick iridium film was sputter-coated (model Q150T S/E/ES, Quorum Technologies, Lewes, UK) on the samples before viewing.
4
Characterization of LBG Microparticles
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The surface morphology of LBG microparticles was characterised by field emission scanning electron microscopy (FESEM; FESEM Ultra Plus, Zeiss, Germany). Dry powders were placed onto metal plates and 5 nm thick iridium film was sputter-coated (model Q150T S/E/ES, Quorum Technologies, UK) on the samples before viewing.
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