Pco edge scmos version 4.2 cl hs cameras

Manufactured by Zeiss

The Pco.edge sCMOS (version 4.2 CL HS) cameras are high-performance scientific CMOS (sCMOS) cameras designed for demanding imaging applications. They feature a 2048 x 2048 pixel sensor with a pixel size of 6.5 μm and a quantum efficiency of up to 82%. The cameras offer a maximum frame rate of 100 frames per second and a dynamic range of up to 15 bits. They support both USB 3.0 and Camera Link HS interfaces for flexible integration into various systems.

Automatically generated - may contain errors

Lab products found in correlation

Plan apochromat 63 1.4 oil dic m27 objective, by Zeiss (2 mentions) Elyra 7 axioobserver microscope, by Zeiss (2 mentions)

Close spelling variants of this product

Pco.edge sCMOS camera SCMOS camera

2 protocols using pco edge scmos version 4.2 cl hs cameras

Structured Illumination Microscopy Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Lattice-SIM images were acquired on a Zeiss Elyra 7 AxioObserver microscope equipped with an Plan-Apochromat 63×/1.4 Oil DIC M27 objective and two pco.edge sCMOS (version 4.2 CL HS) cameras. The system contained 405 nm and 642 nm diode, and 488 nm and 561 nm OPSL lasers. For each focal plane 13 phase images were acquired. Exposure time and laser power were balanced for each fluorescence channel individually to minimize bleaching and exposure time. SIM and Lattice-SIM reconstruction was performed with the SIM processing Tool of the ZEN 3.0 SR (black) software.
Information on the specific hardware configurations and acquisition settings of all microscopy platforms are available on request.

Tröger J., Hoischen C., Perner B., Monajembashi S., Barbotin A., Löschberger A., Eggeling C., Kessels M.M., Qualmann B, & Hemmerich P. (2020). Comparison of Multiscale Imaging Methods for Brain Research. Cells, 9(6), 1377.

+ Open protocol

+ Expand

Lattice SIM Microscopy Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Lattice-SIM images were acquired on a Zeiss Elyra 7 AxioObserver microscope equipped with an Plan-Apochromat 63×/1.4 Oil DIC M27 objective and two pco.edge sCMOS (version 4.2 CL HS) cameras which were aligned at the start of the experiment daily. The system contained 405 nm and 642 nm diode, and 488 nm and 561 nm OPSL lasers. For each focal plane 13 phase images were acquired. Exposure time and laser power were balanced for each fluorescence channel individually to minimize bleaching and set to values between 20–50 ms and 1–1.5 %, respectively. Alexa 488, 568 and 647 were excited using LBF 405/488/561/642 filter set and the emission was captured through an SBP 490–560 + LP 640 emission filter. SIM grid size was 36.5μm and SIM image processing and reconstruction was performed with the SIM processing tool of the ZEN 3.0 SR (black) software (Zeiss). Stacks were processing using 3D mode with default settings and channel alignment was apply to output images.

Muñoz-Estrada J., Nguyen A.V, & Goetz S.C. (2023). TTBK2 mutations associated with spinocerebellar ataxia type 11 disrupt peroxisome dynamics and ciliary localization of SHH signaling proteins. bioRxiv.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!