Briefly, cells (BxPC-3, 3×104 cells/well and KP-4, 2×104 cells/well) were seeded into CELLview 35-mm glass-bottom cell culture dishes with four compartments. Then, 2 days after seeding, the culture medium was replaced with fresh medium containing 5 µM Fluo-8-AM (AAT Bioquest, Inc.) and cultured at 37°C for 1 h. After washing, the cells were treated with a combination of lapatinib (10 µM) and FTY720 (5 µM) at 37°C for 1 h. Cells were then visualized using an LSM 700 confocal laser scanning fluorescence microscope (Zeiss GmbH) equipped with a Plan-Apochromat 40×/1.4 oil DIC (Zeiss GmbH). All images were acquired and processed using ZEN 2012. The object-based fluorescence intensity was measured using ImageJ software.
Plan apochromat 40 1.4 oil dic
Manufactured by Zeiss
The Plan-Apochromat 40×/1.4 oil DIC is a high-performance objective lens designed for microscopy applications. It features a magnification of 40x and a numerical aperture of 1.4, providing exceptional optical performance and resolution. The lens is optimized for use with oil immersion and employs a Plan-Apochromat design, ensuring flat field and accurate color reproduction across the entire field of view. The DIC (Differential Interference Contrast) feature enables enhanced contrast and visualization of fine details in samples.
Automatically generated - may contain errors
Lab products found in correlation
Lsm 700, by Zeiss (2 mentions)
Lsm 710, by Zeiss (2 mentions)
Zen blue software, by Zeiss (2 mentions)
Lsm 700 confocal laser scanning fluorescence microscope, by Zeiss (1 mentions)
Fluo 8 am, by AAT Bioquest (1 mentions)
Lsm 880 airyscan, by Zeiss (1 mentions)
Zen black software, by Zeiss (1 mentions)
Plan apochromat 20 0.8 m27, by Zeiss (1 mentions)
Lsm780 confocal laser scanning microscope, by Zeiss (1 mentions)
3 protocols using plan apochromat 40 1.4 oil dic
1
Calcium Imaging of Cell Response
2
Laser Scanning Confocal Imaging Protocol
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Laser scanning confocal imaging was carried out using the Zeiss LSM700 confocal microscope using the 40×/1.3 Oil DIC Plan Apochromat, M27 objective lens with the diode 405 nm, 488 nm, 55 nm and 639 nm lasers or the Zeiss LSM880 + Airyscan upright confocal microscope using the Plan-Apochromat 40×/1.4 Oil DIC objective lens with the diode 405 nm, argon 488 nm, DPSS 561 nm and HeNe 633 nm lasers. Image analysis was carried out in Fiji Is Just ImageJ (FIJI).
3
Fluorescence Microscopy Imaging Protocol
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Fluorescence microscopy was performed using a LSM710 or LSM780 Confocal Laser Scanning Microscope (Zeiss) at room temperature equipped with a 20× water-immersion (Plan-Apochromat” 20×/0,8 M27, Zeiss), a 40× water-immersion (Objective LD C-Apochromat 40×/1.1 W Corr M27, Zeiss) or a 40× oil-immersion (Plan-Apochromat 40×/1,4 Oil DIC, Zeiss, at LSM 780) objective lens. Images were acquired using ZEN Black software (Zeiss), final adjustments for brightness were done using either ZEN blue software (Zeiss) or ImageJ (mouse SHP1 staining). All images can be made available upon request.
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