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Goat anti foxa2

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Goat anti-FOXA2 is a primary antibody that recognizes the FOXA2 protein. FOXA2 is a forkhead box protein that functions as a transcription factor involved in embryonic development and regulation of gene expression.

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Lab products found in correlation

Ecl western blotting detection reagent, by Cytiva (2 mentions) Rabbit anti foxa1, by Cell Signaling Technology (2 mentions) Mouse anti actb, by Cell Signaling Technology (2 mentions) Rabbit anti foxa2, by Cell Signaling Technology (2 mentions) Nitrocellulose pre cut blotting membranes, by Thermo Fisher Scientific (2 mentions) Alexa 647 conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions) Rabbit anti th, by Merck Group (1 mentions) Lsm 980 airyscan microscope, by Zeiss (1 mentions)

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FOXA2 (9 citations)

3 protocols using goat anti foxa2

1

Western Blot Analysis of FOXA Transcription Factors

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Cells were harvested and lysed with cell lysis buffer (Cell Signaling Technology, 9803) containing protease inhibitor (Roche, 05892791001) and 1 mM PMSF (MP Biomedicals, ICN19538105). Sample preparation follows NuPAGE Novex protocol. 10% Bis-Tris Gel (Novex, NP0301BOX) was used and transferred to Nitrocellulose Pre-Cut Blotting Membranes (Novex, LC2001). Blocking was performed with 5% milk in Tris-based saline with 0.1% Tween 20 (TBST) for 30 min at RT. After blocking, primary antibodies were diluted in blocking solution and incubated overnight at 4°C. The following antibodies were used with noted dilution ratios: goat anti-FOXA2 (R&D, AF2400, 1:1000), rabbit anti-FOXA2 (Cell Signaling Technology, 3143S, 1:1000), rabbit anti-FOXA1 (Cell Signaling Technology, 58613S, 1:1000), mouse anti-ACTB (Cell Signaling Technology, 3700S, 1:10,000). After washing with TBST, HRP conjugated secondary antibodies in blocking solution were incubated at RT for 1 hour and the gel was visualized using ECL western blotting detection reagent (Amersham, RPN2236). Antibodies for this study are summarized in Table S8.
Lee K., Cho H., Rickert R.W., Li Q.V., Pulecio J., Leslie C.S, & Huangfu D. (2019). FOXA2 Is Required for Enhancer Priming during Pancreatic Differentiation. Cell reports, 28(2), 382-393.e7.
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2

Immunostaining of Dopaminergic Neuronal Markers

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Cells were fixed by 4% paraformaldehyde (PFA) for 30 min at 4°C, pre-incubated with 5% donkey serum in PBS with 0.1% Triton X-100 (PBST) for 1 hr, incubated with primary antibodies goat anti-FOXA2 (1:1000, R&D Systems), rabbit anti-LMX1 (1:2000, Millipore), mouse anti-Nurr1 (1:1000, Perseus Proteomics), or rabbit anti-TH (1:1000, Millipore) at 4°C overnight, followed by Alexa488, Alexa555 or Alexa647-conjugated secondary antibodies (Invitrogen) for 1 hr and then 4’,6-diamidino-2-phenylindole (DAPI) for 15 min. Images were visualized in a Zeiss LSM 980 Airyscan microscope (Figure S6L).
Pantazis C.B., Yang A., Lara E., McDonough J.A., Blauwendraat C., Peng L., Oguro H., Kanaujiya J., Zou J., Sebesta D., Pratt G., Cross E., Blockwick J., Buxton P., Kinner-Bibeau L., Medura C., Tompkins C., Hughes S., Santiana M., Faghri F., Nalls M.A., Vitale D., Ballard S., Qi Y.A., Ramos D.M., Anderson K.M., Stadler J., Narayan P., Papademetriou J., Reilly L., Nelson M.P., Aggarwal S., Rosen L.U., Kirwan P., Pisupati V., Coon S.L., Scholz S.W., Priebe T., Öttl M., Dong J., Meijer M., Janssen L.J., Lourenco V.S., van der Kant R., Crusius D., Paquet D., Raulin A.C., Bu G., Held A., Wainger B.J., Gabriele R.M., Casey J.M., Wray S., Abu-Bonsrah D., Parish C.L., Beccari M.S., Cleveland D.W., Li E., Rose I.V., Kampmann M., Aristoy C.C., Verstreken P., Heinrich L., Chen M.Y., Schüle B., Dou D., Holzbaur E.L., Zanellati M.C., Basundra R., Deshmukh M., Cohen S., Khanna R., Raman M., Nevin Z.S., Matia M., Van Lent J., Timmerman V., Conklin B.R., Chase K.J., Zhang K., Funes S., Bosco D.A., Erlebach L., Welzer M., Kronenberg-Versteeg D., Lyu G., Arenas E., Coccia E., Sarrafha L., Ahfeldt T., Marioni J.C., Skarnes W.C., Cookson M.R., Ward M.E, & Merkle F.T. (2022). A reference human induced pluripotent stem cell line for large-scale collaborative studies. Cell stem cell, 29(12), 1685-1702.e22.
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3

Western Blot Analysis of FOXA Transcription Factors

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were harvested and lysed with cell lysis buffer (Cell Signaling Technology, 9803) containing protease inhibitor (Roche, 05892791001) and 1 mM PMSF (MP Biomedicals, ICN19538105). Sample preparation follows NuPAGE Novex protocol. 10% Bis-Tris Gel (Novex, NP0301BOX) was used and transferred to Nitrocellulose Pre-Cut Blotting Membranes (Novex, LC2001). Blocking was performed with 5% milk in Tris-based saline with 0.1% Tween 20 (TBST) for 30 min at RT. After blocking, primary antibodies were diluted in blocking solution and incubated overnight at 4°C. The following antibodies were used with noted dilution ratios: goat anti-FOXA2 (R&D, AF2400, 1:1000), rabbit anti-FOXA2 (Cell Signaling Technology, 3143S, 1:1000), rabbit anti-FOXA1 (Cell Signaling Technology, 58613S, 1:1000), mouse anti-ACTB (Cell Signaling Technology, 3700S, 1:10,000). After washing with TBST, HRP conjugated secondary antibodies in blocking solution were incubated at RT for 1 hour and the gel was visualized using ECL western blotting detection reagent (Amersham, RPN2236). Antibodies for this study are summarized in Table S8.
Lee K., Cho H., Rickert R.W., Li Q.V., Pulecio J., Leslie C.S, & Huangfu D. (2019). FOXA2 Is Required for Enhancer Priming during Pancreatic Differentiation. Cell reports, 28(2), 382-393.e7.
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