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Goat anti mouse igg hrp a0216

Manufactured by Beyotime
Sourced in China

Goat anti-mouse IgG-HRP (A0216) is a laboratory reagent used for the detection of mouse immunoglobulin G (IgG) in various immunoassays. It contains goat-derived antibodies that are conjugated to the enzyme horseradish peroxidase (HRP).

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2 protocols using goat anti mouse igg hrp a0216

1

Platelet Activation and Signaling Assays

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BAPTA-AM, AIM2-IN-3, Ac-IETD-CHO, Belancasan, Ac-DEVE-CHO and Necrosulfon were purchased from MCE. Thrombin and fibrillar type I collagen were purchased from Sigma. CD62P and αIIbβ3 integrin (Wug.E9, JON/A-PE, D200) were purchased from BIOHUB. NINJ1 antibody (sc-136295) was purchased from SCBIO. Goat anti-rabbit IgG-HRP (A0208) and goat anti-mouse IgG-HRP (A0216) were purchased from Beyotime Biotechnology.
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2

Adipogenesis Pathway Protein Analysis

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Antibodies against β-actin (#4967), AMPKα (#5832), and phospho-AMPKα at Thr172 (#2535) were purchased from Cell Signaling Technology (Danvers, MA, USA). Antibodies against FABP4 (ab92501), PPARγ (ab41928), PGC1α (ab54481), PRDM16 (ab106410), UCP1 (ab10983), and PPARα (ab3484) were purchased from Abcam (Cambridge, UK). Goat anti-rabbit IgG HRP (A0208) and goat anti-mouse IgG HRP (A0216) secondary antibodies were bought from Beyotime Institute of Biotechnology (Haimen, Jiangsu, China).
Insulin (91077C), indomethacin (I7378), dexamethasone (D4902), PA (P4060), 3-isobutyl-1-methylxanthine (I5878), triiodothyronine (T3) (I2877), DMSO (D2650), Polybrene (H9268) and Oil-Red O (O0625) were purchased from Sigma (St Louis, MO, USA). DMEM (11960–044) and Pierce™ ECL Western Blotting Substrate (#32109) were purchased from Thermo Fisher Scientific (Waltham, MA, USA). GW6471 (4618) and SR59230A (1511) were purchased from Tocris Bioscience (Avonmouth, Bristol, UK). Mito Stress Test Kit (103015–100) was purchased from Agilent Technologyies (Wilmington, USA).
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