We obtained the tissue microarray from the Outdo Biotech company (HLugA180Su08, Shanghai, China) and the ethics was approved. Anti‐CFL1 antibody (rabbit, 10960‐1‐AP), anti‐PABPC1 antibody (rabbit, 10970‐1‐AP), anti‐CCDC85B antibody (rabbit, 18282‐1‐AP, Proteintech, China), anti‐PFN1 (mouse, 67390‐1‐Ig, Proteintech, China), anti‐HSP90AA1 (rabbit, 13171‐1‐AP, Proteintech, China), anti‐HMGB1 (rabbit, 10829‐1‐AP, Proteintech, China) and anti‐RPS15 (rabbit, 14957‐1‐AP, Proteintech, China) were used as the primary antibody. The secondary antibody (GB23301, GB23303, Servicebio, China) was subsequently used for incubation and tyramide signal amplification (TSA) (FITC‐TSA, CY3‐TSA, 594‐TSA, 647‐TSA, Servicebio, China). The nuclei were stained with 4′,6‐diamidino2‐phenylindole dihydrochloride (DAPI). The Pannoramic Scanner (3D HISTECH, Hungary) was used for image capture. The quantification of the stained markers was performed.
Anti hsp90aa1
Manufactured by Proteintech
Sourced in United States
Anti-HSP90AA1 is a primary antibody that recognizes the heat shock protein 90 alpha, class A member 1 (HSP90AA1). HSP90AA1 is a molecular chaperone protein involved in the folding and stabilization of various client proteins. The anti-HSP90AA1 antibody can be used to detect and study the expression and localization of HSP90AA1 in various biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti c myc, by Cell Signaling Technology (4 mentions)
Anti skp1, by Cell Signaling Technology (4 mentions)
Anti fbxl6, by Merck Group (4 mentions)
Anti cul1, by Santa Cruz Biotechnology (4 mentions)
Anti gapdh, by Cell Signaling Technology (4 mentions)
Anti flag m2, by Merck Group (4 mentions)
Anti pfn1, by Proteintech (1 mentions)
Pannoramic scanner, by 3DHISTECH (1 mentions)
Anti hmgb1, by Proteintech (1 mentions)
Hluga180su08, by Shanghai Outdo Biotech Co. (1 mentions)
5 protocols using anti hsp90aa1
1
Quantitative Multiplex Immunofluorescence Tissue Proteomics
2
Western Blot Analysis of Protein Expression
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Cells were lysed with lysis buffer (100 mM Tris-HCl, pH 6.8, 100 mM DTT, 1% SDS, 10% glycerol). Proteins were separated by 10–12% SDS-PAGE, and transferred to NC membrane. Membranes were blocked in 5% non-fat milk in phosphate-buffered saline (PBS) for 1 h before incubation with primary antibody overnight at 4 °C. Membranes were washed with and incubated with secondary antibody for 1 h. Primary antibodies used as indicated: anti-Flag M2 (1:4000 dilution, F1804, Sigma), anti-HSP90AA1 (1:1000 dilution, 13,171–1-AP, Protein tech), anti-FBXL6 (1:1000 dilution, SAB1407299, Sigma), anti-Cul1 (1:1000 dilution, sc-17,775, Santa cruz, U.S.A), anti-SKP1 (1:2000 dilution, #12248, Cell Signaling Technology, U.S.A), anti-c-Myc (1:1000 dilution, #18583, Cell Signaling Technology, U.S.A), and anti-GAPDH (1:5000 dilution, #5174, Cell Signaling Technology, U.S.A).
3
Western Blot Analysis of Protein Interactions
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Cells were lysed with lysis buffer (100 mM Tris-HCl, pH 6.8, 100 mM DTT, 1% SDS, 10 % glycerol). Proteins were separated by 10-12% SDS-PAGE, and transferred to NC membrane. Membranes were blocked in 5% non-fat milk in phosphate-buffered saline (PBS) for 1 h before incubation with primary antibody overnight at 4 °C. Membranes were washed with and incubated with secondary antibody for 1 h. Primary antibodies used as indicated: anti-Flag M2 (1:4,000 dilution, F1804, Sigma), anti-HSP90AA1
(1:1,000 dilution, 13171-1-AP, Protein tech), anti-FBXL6 (1:1,000 dilution, SAB1407299, Sigma), anti-Cul1 (1:1000 dilution, sc-17775, Santa cruz, U.S.A), anti-SKP1 (1:2,000 dilution, #12248, Cell Signaling Technology, U.S.A), anti-c-Myc (1:1,000 dilution, #18583, Cell Signaling Technology, U.S.A), and anti-GAPDH (1:5,000 dilution, #5174, Cell Signaling Technology, U.S.A).
(1:1,000 dilution, 13171-1-AP, Protein tech), anti-FBXL6 (1:1,000 dilution, SAB1407299, Sigma), anti-Cul1 (1:1000 dilution, sc-17775, Santa cruz, U.S.A), anti-SKP1 (1:2,000 dilution, #12248, Cell Signaling Technology, U.S.A), anti-c-Myc (1:1,000 dilution, #18583, Cell Signaling Technology, U.S.A), and anti-GAPDH (1:5,000 dilution, #5174, Cell Signaling Technology, U.S.A).
4
Western Blot Analysis of Protein Interactions
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells were lysed with lysis buffer (100 mM Tris-HCl, pH 6.8, 100 mM DTT, 1% SDS, 10 % glycerol). Proteins were separated by 10-12% SDS-PAGE, and transferred to NC membrane. Membranes were blocked in 5% non-fat milk in phosphate-buffered saline (PBS) for 1 h before incubation with primary antibody overnight at 4 °C. Membranes were washed with and incubated with secondary antibody for 1 h. Primary antibodies used as indicated: anti-Flag M2 (1:4,000 dilution, F1804, Sigma), anti-HSP90AA1
(1:1,000 dilution, 13171-1-AP, Protein tech), anti-FBXL6 (1:1,000 dilution, SAB1407299, Sigma), anti-Cul1 (1:1000 dilution, sc-17775, Santa cruz, U.S.A), anti-SKP1 (1:2,000 dilution, #12248, Cell Signaling Technology, U.S.A), anti-c-Myc (1:1,000 dilution, #18583, Cell Signaling Technology, U.S.A), and anti-GAPDH (1:5,000 dilution, #5174, Cell Signaling Technology, U.S.A).
(1:1,000 dilution, 13171-1-AP, Protein tech), anti-FBXL6 (1:1,000 dilution, SAB1407299, Sigma), anti-Cul1 (1:1000 dilution, sc-17775, Santa cruz, U.S.A), anti-SKP1 (1:2,000 dilution, #12248, Cell Signaling Technology, U.S.A), anti-c-Myc (1:1,000 dilution, #18583, Cell Signaling Technology, U.S.A), and anti-GAPDH (1:5,000 dilution, #5174, Cell Signaling Technology, U.S.A).
5
Western Blot Analysis of Protein Targets
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Cells were lysed with lysis buffer (100 mM Tris-HCl, pH 6.8, 100 mM DTT, 1% SDS, 10 % glycerol).
Proteins were separated by 10-12% SDS-PAGE, and transferred to NC membrane. Membranes were blocked in 5% non-fat milk in phosphate-buffered saline (PBS) for 1 h before incubation with primary antibody overnight at 4 °C. Membranes were washed with and incubated with secondary antibody for 1 h. Primary antibodies used as indicated: anti-Flag M2 (1:4,000 dilution, F1804, Sigma), anti-HSP90AA1 (1:1,000 dilution, 13171-1-AP, Protein tech), anti-FBXL6 (1:1,000 dilution, SAB1407299, Sigma), anti-Cul1
(1:1000 dilution, sc-17775, Santa cruz, U.S.A), anti-SKP1 (1:2,000 dilution, #12248, Cell Signaling Technology, U.S.A), anti-c-Myc (1:1,000 dilution, #18583, Cell Signaling Technology, U.S.A), and anti-GAPDH (1:5,000 dilution, #5174, Cell Signaling Technology, U.S.A).
Proteins were separated by 10-12% SDS-PAGE, and transferred to NC membrane. Membranes were blocked in 5% non-fat milk in phosphate-buffered saline (PBS) for 1 h before incubation with primary antibody overnight at 4 °C. Membranes were washed with and incubated with secondary antibody for 1 h. Primary antibodies used as indicated: anti-Flag M2 (1:4,000 dilution, F1804, Sigma), anti-HSP90AA1 (1:1,000 dilution, 13171-1-AP, Protein tech), anti-FBXL6 (1:1,000 dilution, SAB1407299, Sigma), anti-Cul1
(1:1000 dilution, sc-17775, Santa cruz, U.S.A), anti-SKP1 (1:2,000 dilution, #12248, Cell Signaling Technology, U.S.A), anti-c-Myc (1:1,000 dilution, #18583, Cell Signaling Technology, U.S.A), and anti-GAPDH (1:5,000 dilution, #5174, Cell Signaling Technology, U.S.A).
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