Dissected tumours were fixed in 4% paraformaldehyde for 48 h and embedded in paraffin blocks. Sections of 4 µm were stained with the following antibodies: Ki67 (Cell Signaling Technology, 12202), P-S5 PolII (Abcam, ab193467), P-S2 PolII (Abcam, ab5095), and P-T161/T160 CDK1/CDK2 (Abcam, ab201008). Staining was visualised using Dako REAL DAB + Chromogen (Agilent Technologies). Slides were counterstained with haematoxylin. Staining was quantified in a blinded fashion in two different fields of view per tumour in three animals per group.
P s2 polii
Manufactured by Abcam
The P-S2 PolII is a laboratory instrument used to study the phosphorylation of the serine 2 residue in the C-terminal domain of RNA polymerase II. It provides a quantitative measure of this specific post-translational modification, which is important for the regulation of gene transcription.
Automatically generated - may contain errors
Lab products found in correlation
P s5 polii, by Abcam (2 mentions)
P t161 t160 cdk1 cdk2, by Abcam (2 mentions)
Dako real dab chromogen, by Agilent Technologies (1 mentions)
Polii, by Abcam (1 mentions)
P s780 rb, by Abcam (1 mentions)
Ps15 p53, by Cell Signaling Technology (1 mentions)
Gapdh, by Cell Signaling Technology (1 mentions)
β actin, by Abcam (1 mentions)
Cleaved parp1, by Abcam (1 mentions)
2 protocols using p s2 polii
1
Immunohistochemical Analysis of Tumour Samples
2
Immunoblotting of Cell Cycle Regulators
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Immunoblotting was carried out as previously described [40 (link)] using the following primary antibodies: CDK7 (Cell Signaling Technology, 2916), CDK1 (Cell Signaling Technology, 9116), CDK2 (Cell Signaling Technology, 2546), CDK4 (Cell Signaling Technology, 12790), CDK9 (Cell Signaling Technology, 2316), β-actin (Abcam, ab6276), GAPDH (Cell Signaling Technology, 2118), P-S5 PolII (Abcam, ab5401), P-S2 PolII (Abcam, ab5095), PolII (Abcam, ab817), P-S780 Rb (Abcam, ab47763), P-S807/811 Rb (Cell Signaling Technology, 8516), Rb (Abcam, ab6075), cyclin H (Abcam, ab54903), MAT1 (Santa Cruz Biotechnology, sc135981), P-S15 p53 (Cell Signaling Technology, 9284), p53 (Santa Cruz Biotechnology, sc-126), P-T161/T160 CDK1/CDK2 (Abcam, ab201008), P-S10 Histone H3 (Abcam, ab139417), AR (MilliporeSigma, 06-680), P-T1457 MED1 (Abcam, ab60950), MED1 (Bethyl Laboratories, A300-793A), cleaved PARP1 (Abcam, ab4830), p21 (Santa Cruz Biotechnology, sc-817).
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