The fruiting body of Hericium erinaceus was purchased from Jilin Jiaohe Songshan Food Co., Ltd. (Jiaohe, China). Dextran standards (670, 410, 270, 80, 25, 12 and 5 kDa) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Monosaccharide standards, including arabinose, mannose, fucose, xylose, rhamnose, galactose and glucose were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Cell Counting Kit-8 was obtained from APExBIO Technology LLC (Houston, TX, USA). Neutral red staining solution, nitric oxide assay kit and BCA kit were obtained from Beyotime Biological Technology Co., Ltd. (Shanghai, China). Western Blot assay kit, SDS-PAGE kit, ELISA kits of TNF-α, IL-6, IL-10 and IFN were obtained from Boster Biological Technology Co., Ltd. (Wuhan, China). Anti-ERK, anti-phospho ERK, anti-JNK, anti-phospho JNK, anti-p38 MAPK, anti-phospho p38 MAPK, anti-Akt, anti-phospho Akt, anti-IkBα, anti-p65, anti-β-actin and anti-Histone H2A were purchased from ABclonal Biotechnology Co., Ltd. (Wuhan, China). Inhibitors of BAY11-7082, SB203580, SP600125, PD98059 and LY294002 were purchased from Selleck Chemicals (Shanghai, China).
Anti phospho erk
Manufactured by ABclonal
Sourced in China, United Kingdom
Anti-phospho ERK is a lab equipment product that detects phosphorylated forms of extracellular signal-regulated kinase (ERK). It is a tool for monitoring the activation status of the ERK signaling pathway.
Automatically generated - may contain errors
Lab products found in correlation
Anti β actin, by ABclonal (2 mentions)
Anti p65, by ABclonal (2 mentions)
Anti phospho jnk, by ABclonal (2 mentions)
Dextran standard, by Merck Group (1 mentions)
Bca kit, by Beyotime (1 mentions)
Galactose, by Merck Group (1 mentions)
Fucose, by Merck Group (1 mentions)
Rhamnose, by Merck Group (1 mentions)
Mannose, by Merck Group (1 mentions)
Xylose, by Merck Group (1 mentions)
Arabinose, by Merck Group (1 mentions)
Pd98059, by Selleck Chemicals (1 mentions)
Nitric oxide assay kit, by Beyotime (1 mentions)
Anti erk, by ABclonal (1 mentions)
Anti p38 mapk, by ABclonal (1 mentions)
Anti phospho akt, by ABclonal (1 mentions)
Anti jnk, by ABclonal (1 mentions)
Ly294002, by Selleck Chemicals (1 mentions)
Bay11 7082, by Selleck Chemicals (1 mentions)
Sp600125, by Selleck Chemicals (1 mentions)
2 protocols using anti phospho erk
1
Hericium erinaceus Extract Bioactivity Profiling
2
Western Blot Immunodetection of Signaling Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
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For the immunodetection, 50 µg of total protein extracts from cytosol or nucleus in Laemmli sample buffer (Bio-Rad, Hercules, USA) were resolved on 10% or 15% sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred to nitrocellulose membranes for western blotting. The membranes were first stained with Ponceau S to confirm the transfer efficacy. After blocking with 5% skim milk dissolved in Tris-buffered saline (TBS) containing 0.05% Tween-20 (TBST) (VWR, Lutterworth, UK) for 2 h at room temperature, membranes were incubated with anti-phospho-ERK, anti-phospho-p38, anti-phospho-JNK, anti-β actin, anti-Histone H3, anti-phospho-IκBα, anti-IκBα, anti-phospho-p65-S536, or anti-p65 (ABclonal, Woburn, USA) at appropriate dilutions, followed by goat anti-rabbit secondary antibody conjugated with horseradish peroxidase (HRP). Positive band intensities were detected using a gel documentation system (Fujifilm LAS-3000 Imager, Tokyo, Japan). 35 (link)
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