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Nanowizard bioafm

Manufactured by Bruker
Sourced in United States

The NanoWizard BioAFM is an atomic force microscope (AFM) designed for biological applications. It provides high-resolution imaging and characterization of biological samples in their native environments.

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2 protocols using nanowizard bioafm

1

Multimodal Analysis of Cell Response

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The fluorescence spectra and the CCK‐8 assay were performed using a Thermo Scientific Varioskan Flash (MA, USA). The fluid mode AFM was carried on NanoWizard BioAFM (Bruker, MA, USA). The DLS was also performed using a Zetasizer Nano ZSP (Malvern, USA). The CLSM imaging was conducted utilizing a ZEISS LSM780 (Zeiss, Germany). The RT‐PCR was analyzed via a CFX96 Real‐Time System (Bio‐Rad, USA). The FCM analysis was performed using a BD FACSCanto II flow cytometer (BD, USA). The in vivo imaging for mice was recorded on IVIS Spectrum CT (PerkinElmer, USA).
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2

Multimodal Biomolecular Analysis Suite

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Polyacrylamide gel electrophoresis (PAGE) was performed on an electrophoresis system and imaged on the ChemiDoc XRS+ (Bio-Rad, CA, USA). Fluid mode atomic force microscopy (AFM) was carried on NanoWizard® BioAFM (Bruker, MA, USA). The colorimetric assays and the fluorescence spectra were recorded using a Thermo Scientific Varioskan Flash (MA, USA). A hemocytometer (Bright Line) was sourced from VWR (Radnor, PA, USA). Surface morphology images of 3D-codes were analyzed using scanning electron microscopy (SEM) (S–3400 N, Tokyo, Japan) and flow counted using luminex 200 (TX, USA). Sequencing was carried out in sequenator (3730XL, ABI, CA, USA).
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