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Mcp 1 df7577

Manufactured by Affinity Biosciences
Sourced in China

MCP-1 (DF7577) is an ELISA kit developed by Affinity Biosciences for the quantitative detection of human Monocyte Chemoattractant Protein-1 (MCP-1) in various biological samples. The kit utilizes the sandwich ELISA technique and contains the necessary components required to measure MCP-1 levels.

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3 protocols using mcp 1 df7577

1

Antioxidant and Anti-inflammatory Potentials of Mineral Waters

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The glyoxylate was provided by Sigma (G4502). GSH (A006-1-1) and MDA (A003-1-2) were from Nanjing Jiancheng Bioengineering Institute. The SOD (E-BC-K020-M) was purchased from Elabscience. The primary antibodies against OPN (22925), CD44 (15675), Nrf2 (16396), HO-1 (10701), and SOD-1 (10269) were purchased from Proteintech Company. Cell Signaling Technology provided primary antibodies against c-c3 (cleaved-caspase-3) (96611) and SIRT1 (8469). Primary antibodies against α-SMA (CY5295) were bought from Abways, and MCP-1 (DF7577) was from Affinity. Alkaline natural mineral water at high pH (pH 9.3, Shilin Tianwaitian) and alkaline natural mineral water at low pH (pH 8.3, Shilin Tianwaitian) were purchased from Jingdong Online Mall.
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2

Eugenol Attenuates Oxidized LDL-Induced Vascular Injury

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Eugenol (purity: 98.11%) was acquired from MedChemExpress (Shanghai, China). Oxidized low-density lipoprotein was purchased from Guangzhou Yiyuan Biotechnology Co., Ltd (Cat. No. YB-002). Valsartan (purity ≥ 98%) was purchased from the Aladdin Chemical Reagent official website. 10% fetal sheep serum, 1% penicillin and streptomycin, low sugar medium, enhanced chemiluminescence (ECL) solution, SevenFast Total RNA Extraction Kit for Cells and SevenFast® Two-step RT-qPCR Kit were purchased from Seven, Beijing, China. MTT was purchased from Sigma-Aldrich in St. Louis, Missouri, United States of America. Rabbit monoclonal antibody GAPDH (380626) was purchased from ZENBIO, Chengdu, China; proliferating cell nuclear antigen (PCNA) (10205-2-AP) and AT1 (25343-1-AP) was purchased from Proteintech, Wuhan, China; MFG-E8 (DF10184) and MCP-1 (DF7577) were purchased from Affinity, Jiangsu, China. The BCA protein content determination kit was purchased from BCA kit, Beyotime Biotechnology, Shanghai, China. The flow cytometry cell cycle kit was purchased from Elabscience (Wuhan, China).
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3

Antioxidant and Anti-inflammatory Potentials of Mineral Waters

Check if the same lab product or an alternative is used in the 5 most similar protocols
The glyoxylate was provided by Sigma (G4502). GSH (A006-1-1) and MDA (A003-1-2) were from Nanjing Jiancheng Bioengineering Institute. The SOD (E-BC-K020-M) was purchased from Elabscience. The primary antibodies against OPN (22925), CD44 (15675), Nrf2 (16396), HO-1 (10701), and SOD-1 (10269) were purchased from Proteintech Company. Cell Signaling Technology provided primary antibodies against c-c3 (cleaved-caspase-3) (96611) and SIRT1 (8469). Primary antibodies against α-SMA (CY5295) were bought from Abways, and MCP-1 (DF7577) was from Affinity. Alkaline natural mineral water at high pH (pH 9.3, Shilin Tianwaitian) and alkaline natural mineral water at low pH (pH 8.3, Shilin Tianwaitian) were purchased from Jingdong Online Mall.
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