Anti-HCRP-1 rabbit polyclonal antibodies were purchased from proteintech (Wuhan, China). Rabbit monoclonal antibodies to MMP-2, MMP-9, p38, phospho-p38, C-Jun amino terminal kinase (JNK), phospho-JNK, MEK1/2, phospho-MEK1/2, c-Raf, phospho-c-Raf, Ras and phospho-Ras were purchased from Cell Signaling Technology (Beverly, MA). Antibodies against EGFR, phospho-EGFR (Tyr1173), ERK and phospho-ERK were from Santa Cruze (CA, USA). Mouse anti-β-actin was from Boster Biotechnology (Wuhan, China). ERK1/2 inhibitor, PD98059 and EGFR inhibitor, AG1478 were from Abcam (Shanghai, China).
Phospho ras
Manufactured by Cell Signaling Technology
Sourced in United States
Phospho-Ras is a laboratory product from Cell Signaling Technology that detects the phosphorylation state of Ras proteins. It is used for Western blotting applications to analyze the activation status of the Ras signaling pathway.
Automatically generated - may contain errors
Lab products found in correlation
Phospho p38, by Cell Signaling Technology (1 mentions)
Phospho c raf, by Cell Signaling Technology (1 mentions)
Phospho erk, by Santa Cruz Biotechnology (1 mentions)
Mouse anti β actin, by Boster Bio (1 mentions)
Phospho jnk, by Cell Signaling Technology (1 mentions)
Phospho mek1 2, by Cell Signaling Technology (1 mentions)
Ag1478, by Abcam (1 mentions)
Pd98059, by Abcam (1 mentions)
Anti hcrp 1 rabbit polyclonal antibodies, by Proteintech (1 mentions)
C raf, by Cell Signaling Technology (1 mentions)
Mmp 9, by Cell Signaling Technology (1 mentions)
Mek1 2, by Cell Signaling Technology (1 mentions)
Phospho akt, by Cell Signaling Technology (1 mentions)
Antibodies against p21, by Cell Signaling Technology (1 mentions)
Phospho foxo3a, by Cell Signaling Technology (1 mentions)
Phospho erk1 2, by Cell Signaling Technology (1 mentions)
2 protocols using phospho ras
1
Antibody Panel for HCRP-1 and MAPK Signaling
2
Western Blot Analysis of Cell Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells of logarithmic growth phase were collected. Total proteins were extracted using radioimmunoprecipitation assay (RIPA) buffer; then protein contents were quantified. Equal amounts of proteins were subjected to SDS-PAGE. Western blotting analysis was performed according to a previously established method. Antibodies against p21, p27, phospho-ERK1/2, ERK1/2, phospho-MEK1/2, MEK1/2, Akt, phospho-Akt, FOXO3a, phospho-FOXO3a, RAS, and phospho-RAS were purchased from Cell Signaling Technology (USA), whereas antibodies against Erbin, Grb2, and GAPDH were obtained from Abcam (Cambridge, MA, USA). GAPDH was used as a protein loading control.
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