P nitrophenyl phosphate pnpp reagent

Manufactured by Merck Group

Sourced in Sao Tome and Principe

P-nitrophenyl phosphate (pNPP) reagent is a colorimetric substrate used for the detection and quantification of phosphatase enzyme activity. The reagent is designed to produce a yellow color upon enzymatic hydrolysis, which can be measured spectrophotometrically.

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Lab products found in correlation

Rosiglitazone, by Cayman Chemical (4 mentions) Nile red, by Merck Group (2 mentions) Human insulin, by Merck Group (2 mentions) Perilipin antibody, by Cell Signaling Technology (2 mentions) Insulin, by Merck Group (2 mentions) Tbt chloride, by Merck Group (2 mentions) Lg100268, by Merck Group (2 mentions) Hx531, by Bio-Techne (2 mentions) 17β estradiol, by Merck Group (1 mentions) Tbbpa, by Merck Group (1 mentions) T0070907, by Bio-Techne (1 mentions) Tributyltin chloride, by Merck Group (1 mentions) Abca1 antibody, by Novus Biologicals (1 mentions) T0901317, by Cayman Chemical (1 mentions) Bexarotene, by LC Laboratories (1 mentions) Sodium tartrate, by Merck Group (1 mentions) T0901317, by Merck Group (1 mentions) Gsk2033, by Merck Group (1 mentions) Triphenylphosphate tphp, by Merck Group (1 mentions) Rosiglitazone rosi, by Cayman Chemical (1 mentions)

Spelling variants of this product

P-nitrophenyl phosphate (490 citations) P-nitrophenyl phosphate (pNPP) (126 citations)

5 protocols using p nitrophenyl phosphate pnpp reagent

Adipocyte Lipid Metabolism Assay

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Rosiglitazone was from Cayman Chemical (Ann Arbor, MI). Perilipin antibody was from Cell Signaling Technology (Danvers, MA). 17β-Estradiol, human insulin, Nile Red, and p-nitrophenyl phosphate (pNPP) reagent were from Sigma-Aldrich (St. Louis, MO). All other reagents were from Thermo Fisher Scientific (Suwanee, GA) unless noted otherwise.

Bragdon B., Burns R., Baker A.H., Belkina A.C., Morgan E.F., Denis G.V., Gerstenfeld L.C, & Schlezinger J.J. (2015). Intrinsic Sex-Linked Variations in Osteogenic and Adipogenic Differentiation Potential of Bone Marrow Multipotent Stromal Cells. Journal of cellular physiology, 230(2), 296-307.

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Adipogenesis Assay Reagents

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Rosiglitazone was from Cayman Chemical (Ann Arbor, MI). DMSO was from American Bioanalytical (Natick, MA). Insulin, Nile Red, p-nitrophenyl phosphate (pNPP) reagent, TBT chloride, TPhT chloride, and TBBPA were from Sigma-Aldrich (St. Louis, MO). MEHP was from TCI America (Portland, OR). METBP was synthesized by AsisChem (Waltham, MA). All other reagents were from Thermo Fisher Scientific (Suwanee, GA).

Watt J, & Schlezinger J.J. (2015). Structurally-diverse, PPARγ-activating environmental toxicants induce adipogenesis and suppress osteogenesis in bone marrow mesenchymal stromal cells. Toxicology, 331, 66-77.

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Adipocyte Lipid Accumulation Assay

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Rosiglitazone and T0901317 were from Cayman Chemical (Ann Arbor, MI). Bexarotene was from LC Laboratories (Woburn, MA). The perilipin antibody was from Cell Signaling Technology (Danvers, MA). The ABCA1 antibody was from Novus Biologicals (Littleton, CO). Human insulin, Nile Red, p-nitrophenyl phosphate (pNPP) reagent, LG100268 and tributyltin chloride were from Sigma-Aldrich (St. Louis, MO). HX531 and T0070907 were from Tocris Bioscience (via R&D Systems, Inc., Minneapolis, MN). All other reagents were from Thermo Fisher Scientific (Suwanee, GA) unless noted. Caution: Tributyltin is hazardous and should be handled carefully.

Baker A.H., Watt J., Huang C.K., Gerstenfeld L.C, & Schlezinger J.J. (2015). Tributyltin engages multiple nuclear receptor pathways and suppresses osteogenesis in bone marrow multipotent stromal cells. Chemical research in toxicology, 28(6), 1156-1166.

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Biochemical Assay for Nuclear Receptor Activity

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Rosiglitazone was from Cayman Chemical (Ann Arbor, MI). DMSO was from American Bioanalytical (Natick, MA). GSK2033, LG100268, T0901317, p-nitrophenyl phosphate (pNPP) reagent, TBT chloride, Gil’s Hematoxilin and sodium tartrate were from Sigma-Aldrich (St. Louis, MO). HX531 was from Tocris Bioscience (Bristol, United Kingdom). All other reagents were from Thermo Fisher Scientific (Suwanee, GA) unless noted.

Watt J., Baker A.H., Meeks B., Pajevic P.D., Morgan E.F., Gerstenfeld L.C, & Schlezinger J.J. (2018). Tributyltin induces distinct effects on cortical and trabecular bone in female C57Bl/6J mice. Journal of cellular physiology, 233(9), 7007-7021.

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In Vitro Chemical Bioassay Protocol

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Rosiglitazone (Rosi) was from Cayman Chemical (Ann Arbor, MI). Dimethyl sulfoxide (DMSO) was from American Bioanalytical (Natick, MA). Insulin, Nile Red, p-nitrophenyl phosphate (pNPP) reagent, LG100268 (LG268), LG100754, tributyltin chloride (TBT), and triphenyl phosphate (TPhP) were from Sigma-Aldrich (St. Louis, MO). Test chemicals (Table 1) also were from Sigma-Aldrich, provided via the National Toxicology Program or ordered directly. LG100754 was from (Bio-techne Corp., Minneapolis, MN). All other reagents were from Thermo Fisher Scientific (Suwanee, GA) unless noted.

Andrews F.V., Kim S.M., Edwards L, & Schlezinger J.J. (2020). Identifying Adipogenic Chemicals: Disparate effects in 3T3-L1, OP9 and primary mesenchymal multipotent cell models. Toxicology in vitro : an international journal published in association with BIBRA, 67, 104904.

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