β arrestin1 2 d24h9 rabbit monoclonal antibody

Anti-p-CKR-5(E11/19), which specifically recognizes phosphoserine at position 349 (a specific GRK phosphorylation site) [12 (link)], anti-CKR-5 (D6), anti-GAPDH MAbs, anti-p-ERK1/2 (Thr 202/Tyr 204), anti-ERK1 (C-16), and Rab5 (FL-215) polyclonal antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). β-Arrestin1/2 (D24H9) rabbit monoclonal antibody, which detects endogenous level of total β-arrestin1 and -2 proteins, shows a single band at 50 KDa, as reported in the datasheet, was purchased from Cell Signaling Technology (Beverly, MA, USA). Secondary HRP-conjugated goat anti-rabbit IgG and goat anti-mouse IgG were purchased from Millipore; secondary FITC-conjugated goat anti-mouse IgG was from ICN Biomedicals (Aurora, OH, USA). Secondary HRP-conjugated anti-rabbit and anti-mouse IgG VeriBlot for IP were from Abcam (Cambridge, UK).

Anti-CKR-5 (D6), anti-p-CKR-5(E11/19) that it is recommended for the detection of Ser349 phosphorylated (a specific GRK phosphorylation site)16 (link), anti-GAPDH MAbs and anti-ERK1 (C-16), anti-p-ERK1/2 (Thr 202/Tyr 204) polyclonal antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). β-Arrestin1/2 (D24H9) Rabbit monoclonal antibody was purchased from Cell Signaling Technology (Beverly, MA) which detects endogenous level of total β-arrestin1 and β-arrestin2 proteins showing a single band only at 50 KDa as reported in the datasheet. PE Mouse anti-Human CD195 (2D7), and PE Mouse IgGs Isotype Control were purchased from BD-Pharmingen (San Diego, CA). Secondary HRP-conjugated goat anti-mouse IgG and goat anti-rabbit IgG were purchased from Millipore; secondary FITC-conjugated goat anti-mouse IgG was from ICN Biomedicals (Aurora, OH). Secondary HRP-conjugated anti-mouse IgGVeriBlot and anti-rabbit IgGVeriBlot for IP were from Abcam (UK). The pool of serum samples from 5 LTNP positive for anti-CCR5 natural antibodies (CCR5 Ab Pos) were: LTNP no. 21, LTNP no. 20, LTNP no. 11, LTNP no. 4, and LTNP no. 22, all of which had been characterized previously21 (link)25 (link). The pool of serum samples used as negative control were taken from LTNP negative for anti-CCR5 antibodies (CCR5 Ab Neg), as previously described21 (link)25 (link).