SCC13 cells (a gift from Paolo Dotto, MGH Cutaneous Biology department, USA) were grown in keratinocyte serum-free medium (K-SFM, Gibco) supplemented with EGF (epidermal growth factor) and bovine pituitary extract based on the manufacturer’s instruction. HSC2 cells (a gift from Cyril Benes, MGH Cancer Center, USA) were grown in DMEM/F-12 medium with 10% Tet system approved FBS (Clontech) and 1% penicillin (100 U/ml)/streptomycin (100 U/ml) (Gibco). Human primary keratinocytes were obtained from CellnTec and were grown in CnT-PR medium (CellnTec) supplemented with 1% penicillin (100 U/ml)/streptomycin (100 U/ml) (Gibco). Human tumor-associated fibroblasts (a gift from Salvador Aznar Benitah, IRB, Spain) were grown in DMEM medium supplemented with 10% FBS (Sigma), Insulin-Transferrin-Selenium reagent (Gibco), and 1% penicillin (100 U/ml)/streptomycin (100 U/ml) (Gibco). All cells were cultured and maintained at 37°C under 5% CO2. Human primary keratinocytes (Cat. #HPEKp, CellIn Tec), were passaged with accutase (Gibco) and were used within four passages. All the other cell lines were passaged by trypsinization.
Insulin transferrin selenium reagent
Manufactured by Thermo Fisher Scientific
Sourced in Spain
The Insulin-Transferrin-Selenium reagent is a cell culture supplement that provides essential nutrients for cell growth and maintenance. It contains a combination of insulin, transferrin, and selenium, which are important components for supporting cell proliferation and survival in vitro.
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2 protocols using insulin transferrin selenium reagent
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Cell Culture Protocols for Various Cell Lines
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Cell Culture Protocols for Various Cell Lines
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SCC13 cells (a gift from Paolo Dotto, MGH Cutaneous Biology department, USA) were grown in keratinocyte serum-free medium (K-SFM, Gibco) supplemented with EGF (epidermal growth factor) and bovine pituitary extract based on the manufacturer’s instruction. HSC2 cells (a gift from Cyril Benes, MGH Cancer Center, USA) were grown in DMEM/F-12 medium with 10% Tet system approved FBS (Clontech) and 1% penicillin (100 U/ml)/streptomycin (100 U/ml) (Gibco). Human primary keratinocytes were obtained from CellnTec and were grown in CnT-PR medium (CellnTec) supplemented with 1% penicillin (100 U/ml)/streptomycin (100 U/ml) (Gibco). Human tumor-associated fibroblasts (a gift from Salvador Aznar Benitah, IRB, Spain) were grown in DMEM medium supplemented with 10% FBS (Sigma), Insulin-Transferrin-Selenium reagent (Gibco), and 1% penicillin (100 U/ml)/streptomycin (100 U/ml) (Gibco). All cells were cultured and maintained at 37°C under 5% CO2. Human primary keratinocytes (Cat. #HPEKp, CellIn Tec), were passaged with accutase (Gibco) and were used within four passages. All the other cell lines were passaged by trypsinization.
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