Spss statistics version 26.0 for mac

All analyses were conducted using SPSS statistics version 26.0 for Mac (SPSS, Chicago, IL). Egg hatch proportions were characterized per female (i.e. individual eggs were not treated as independent data points) and this trait as well as longevity were not normally distributed according to Shapiro–Wilk tests; therefore, we analysed these data with nonparametric log‐rank tests for longevity and Kruskal–Wallis for egg hatch proportions. The development time and wing length for females and males were measured by one‐way ANOVAs. Fecundity and Wolbachia density under heat and cold were analysed by two‐way ANOVAs. Wolbachia density for different life stages was compared with a two‐way ANOVA and t test. DENV‐2 TCID₅₀ comparisons across strains were undertaken using a Mann‐Whitney test and differences in the infection rate were evaluated using two‐tailed Fisher's tests. Because we undertook treatment/line comparisons at the same time in randomized designs, there were no experimental block/repeat effects in the analysis.