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Col18a1

Manufactured by Thermo Fisher Scientific
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Col18a1 is a laboratory product used for research purposes. It is a gene that encodes the alpha-1 chain of type XVIII collagen, a component of basement membranes. The core function of Col18a1 is to provide structural support and contribute to the integrity of extracellular matrix.

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Lab products found in correlation

Col6a5, by Thermo Fisher Scientific (2 mentions) Col6a3, by Thermo Fisher Scientific (2 mentions) Col4a1, by Thermo Fisher Scientific (2 mentions) Gapdh, by Thermo Fisher Scientific (2 mentions) Iq sybr green supermix, by Bio-Rad (2 mentions) Icycler, by Bio-Rad (2 mentions) Iscript, by Bio-Rad (2 mentions) Serpine1, by Abcam (1 mentions) Axio imager m1 microscope, by Zeiss (1 mentions) Alexa flour 488, by Abcam (1 mentions) 4 6 diamidino 2 phenylindole dapi solution, by Thermo Fisher Scientific (1 mentions) Alexa flour 555, by Abcam (1 mentions) Pecam 1, by Santa Cruz Biotechnology (1 mentions)

3 protocols using col18a1

1

Immunofluorescence Analysis of Pancreatic Tissue

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Cells were fixed with 4% FA solutions and paraffin-embedded pancreatic tissues were deparaffinized. Fixed cells and tissues were permeabilized with Triton X-100, sequentially incubated with blocking solution and primary antibodies, including Col18a1 (Invitrogen™), Serpin E1, CD68 (Abcam Plc.), NG2 (Sigma-Aldrich), PECAM-1 (Santa Cruz Biotechnology, Inc.), and MPO (Agilent Technologies, lnc., Santa Clara, CA, USA), at 4 °C overnight, and further incubated with secondary antibodies conjugated with Alexa Flour® 488 or Alexa Flour® 555 (Abcam Plc.). DAPI (4’,6-diamidino-2-phenylindole) solution (Invitrogen™) was used to stain the nuclei. Fluorescence signals were observed and imaged using the ZEISS Axio Imager M1 microscope (Carl Zeiss, Oberkochen, Germany).
Jung M., Ryu S., Kim C., Cha S., Kang H., Ji E., Hong Y., Lee Y., Han S., Jeong S.M., Kim W, & Lee E.K. (2022). RNA binding protein HuD mediates the crosstalk between β cells and islet endothelial cells by the regulation of Endostatin and Serpin E1 expression. Cell Death & Disease, 13(12), 1019.
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2

Quantitative Real-Time PCR Analysis

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Total RNA was isolated and first strand cDNA was synthesized using iScript (Bio-Rad). Quantitative real-time PCR with a BioRad iCycler® used primers mixed with the iQ™SYBR®Green Supermix (Bio-Rad). Primer sets for Vegfr1, Vegfr2, Tie2, Itg1, Vegf-variants, Mmp2, Mmp9, Timp1, Timp2, Timp3, Col1a1, Col6a5, Col6a3, Col6a1/2, Col18a1, Col4a1, Lama5 and Gapdh were purchased from Invitrogen. Primers are listed in Supplementary Table 1. Specificity of reactions was determined by melting curve analysis. The relative fold changes of gene expression between each gene of interest were calculated by standard curve method. For each gene of interest ≥3 independent experiments were performed and each experiment was run in duplicates or triplicates.
Besschetnova T.Y., Ichimura T., Katebi N., St. Croix B., Bonventre J.V, & Olsen B.R. (2015). Regulatory mechanisms of anthrax toxin receptor 1-dependent vascular and connective tissue homeostasis. Matrix biology : journal of the International Society for Matrix Biology, 42, 56-73.
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3

Quantitative Real-Time PCR Analysis

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Total RNA was isolated and first strand cDNA was synthesized using iScript (Bio-Rad). Quantitative real-time PCR with a BioRad iCycler® used primers mixed with the iQ™SYBR®Green Supermix (Bio-Rad). Primer sets for Vegfr1, Vegfr2, Tie2, Itg1, Vegf-variants, Mmp2, Mmp9, Timp1, Timp2, Timp3, Col1a1, Col6a5, Col6a3, Col6a1/2, Col18a1, Col4a1, Lama5 and Gapdh were purchased from Invitrogen. Primers are listed in Supplementary Table 1. Specificity of reactions was determined by melting curve analysis. The relative fold changes of gene expression between each gene of interest were calculated by standard curve method. For each gene of interest ≥3 independent experiments were performed and each experiment was run in duplicates or triplicates.
Besschetnova T.Y., Ichimura T., Katebi N., St. Croix B., Bonventre J.V, & Olsen B.R. (2015). Regulatory mechanisms of anthrax toxin receptor 1-dependent vascular and connective tissue homeostasis. Matrix biology : journal of the International Society for Matrix Biology, 42, 56-73.
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