Rabbit polyclonal anti inos antibody

Manufactured by Santa Cruz Biotechnology

Sourced in Japan

The Rabbit polyclonal anti-iNOS antibody is a laboratory tool used to detect the presence and expression levels of the inducible nitric oxide synthase (iNOS) protein in biological samples. This antibody is produced in rabbits and recognizes the iNOS enzyme, which plays a key role in the production of nitric oxide, an important signaling molecule in various physiological and pathological processes.

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Lab products found in correlation

Lsm 780, by Zeiss (2 mentions) Goat polyclonal anti iba 1 antibody, by Abcam (1 mentions) Fv10i w, by Olympus (1 mentions) Anti iba1 rabbit polyclonal antibody, by Fujifilm (1 mentions) Mouse monoclonal anti 3 nitrotyrosine, by Abcam (1 mentions) Rabbit polyclonal anti p2x7r, by Alomone (1 mentions) Pyrrolidine dithiocarbamate, by Merck Group (1 mentions) Apocynin, by Merck Group (1 mentions) Sodium nitrite, by Merck Group (1 mentions) Tnf α, by Merck Group (1 mentions) Lipofectamine rnaimax reagent, by Thermo Fisher Scientific (1 mentions) Phorbol myristate acetate (pma), by Merck Group (1 mentions)

Close spelling variants of this product

Anti-iNOS (77 citations) Rabbit anti-iNOS (16 citations) Anti-iNOS antibody (10 citations) INOS antibody (9 citations) Polyclonal rabbit antibody (4 citations) Polyclonal rabbit (4 citations) Rabbit polyclonal anti-iNOS (3 citations) Rabbit anti-TM (2 citations) Rabbit anti-mouse iNOS polyclonal antibody (2 citations)

2 protocols using rabbit polyclonal anti inos antibody

Immunohistochemical Analysis of P2X7R and Oxidative Markers

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Antigen retrieval was performed by heat treatment in a microwave oven for 21 min in Tris–EDTA buffer solution (0.05 mol/L Tris, 0.001 mol/L EDTA; pH 8.5). Sections were incubated for 30 min in 5 % bovine serum albumin (BSA) and then incubated at 4 °C overnight with primary antibodies (rabbit polyclonal anti-P2X7R, 1:500, Alomone labs; mouse monoclonal anti-3-Nitrotyrosine, 1:400, Abcam; rabbit polyclonal anti-nitrotyrosine antibody, 1:200, Millipore; rabbit polyclonal anti-MPO antibody, 1:50, Abcam; rabbit polyclonal anti-iNOS antibody, 1:40, Santa Cruz Biotechnology; rabbit polyclonal anti-Iba-1 antibody, 1:600, WAKO, Osaka, Japan; goat polyclonal anti-Iba-1 antibody, 1:300, Abcam; mouse monoclonal gp91^phox antibody, 1:400, BD Transduction Laboratories). For double-staining experiments, primary antibodies were separately incubated overnight at 4 °C. After they were washed with phosphate-buffered saline (PBS), sections were then incubated with secondary antibodies. Images were obtained using confocal microscopes (FV10i-W, Olympus, Tokyo, Japan; LSM780, Zeiss, Oberkochen, Germany).

Feng L., Chen Y., Ding R., Fu Z., Yang S., Deng X, & Zeng J. (2015). P2X7R blockade prevents NLRP3 inflammasome activation and brain injury in a rat model of intracerebral hemorrhage: involvement of peroxynitrite. Journal of Neuroinflammation, 12, 190.

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Microglial BV2 cell line protocol

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The murine microglial BV2 cell line was obtained from the neuropharmacology lab, NIEHS (Research Triangle Park, NC). Coumarin-7-boronic acid (CBA) was synthesized as described earlier [18 (link)]. LPS (Escherichia coli 0111:B4) was purchased from Calbiochem (La Jolla, CA) and was reconstituted in ultrapure water and kept at 4°C. Apocynin, L-ω-nitroarginine methyl ester (L-NAME), N-3-(aminomethyl)benzylacetamide.2HCl (1400W), paraformaldehyde, phorbolmyristate acetate (PMA), pyrrolidine dithiocarbamate (PDTC), 5,10,15,20-tetrakis(4-sulfonatophenyl) porphyrinato iron (III) chloride (FeTPPS), SB 202190, sodium nitrite, and TNFα were from Sigma (St. Louis, MO). DMPO was obtained from Dojindo Laboratories (Rockville, MD) and used without further purification. Chicken polyclonal anti-DMPO antibodies were developed in our laboratory and used in the immuno-spin-trapping studies. Rabbit polyclonal anti-iNOS antibody and siRNA against iNOS were from Santa Cruz Biotechnology (Dallas, TX). Rabbit polyclonal anti-3-nitro tyrosine was from Abcam (Cambridge, MA). Lipofectamine RNAiMAX reagent and transfection media were from Invitrogen (Grand Island, NY).

Kumar A., Chen S.H., Kadiiska M.B., Hong J.S., Zielonka J., Kalyanaraman B, & Mason R.P. (2014). Inducible nitric oxide synthase is key to peroxynitrite-mediated, LPS-induced protein radical formation in murine microglial BV2 cells. Free radical biology & medicine, 0, 51-59.

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