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Pi apoptosis detection kit

Manufactured by Dojindo Laboratories
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Sourced in Japan, China

The PI) Apoptosis Detection Kits from Dojindo Laboratories are designed to detect and quantify apoptosis, a programmed cell death process. The kits utilize the fluorescent dye propidium iodide (PI) to stain the nucleic acids of cells, allowing for the identification of apoptotic cells through flow cytometry analysis.

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Lab products found in correlation

Annexin 5, by Dojindo Laboratories (1 mentions) Flow cytometry, by Beckman Coulter (1 mentions) Flow cytometry, by FlowJo (1 mentions) Annexin 5 fluorescein isothiocyanate, by Dojindo Laboratories (1 mentions)

4 protocols using pi apoptosis detection kit

1

Annexin V-FITC/PI Apoptosis Assay

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flow cytometry assay was carried out to detect cell apoptosis. In brief, HT‐1376 and T24 cells transfected with different vectors were harvested and subjected to apoptosis detection using Annexin V(FITC)/Propidium iodide (PI) Apoptosis Detection Kits (Dojindo). Cell apoptosis rate was detected by using flow cytometry (Beckman Coulter) and analyzed using FlowJo 7.6 software (Tree Star, lnc).
Chen Y., Zhang W., Kadier A., Zhang H, & Yao X. (2020). MicroRNA‐769‐5p suppresses cell growth and migration via targeting NUSAP1 in bladder cancer. Journal of Clinical Laboratory Analysis, 34(5), e23193.
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2

Apoptosis Evaluation by Flow Cytometry

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flow cytometry assay was carried out for cell apoptosis assessment. In brief, different treated A549 and H1975 cells were harvested and subjected to apoptosis test using Annexin V (FITC)/propidium iodide (PI) Apoptosis Detection Kits (Dojindo, Japan). Cell apoptosis rate was detected by using flow cytometry (Beckman Coulter, CA, U.S.A.) and analyzed using FlowJo 7.6 software (FlowJo LLC, Ashland, OR, U.S.A.).
Meng J., Liu L., Wang D., Yan Z, & Chen G. (2020). Hydrogen gas represses the progression of lung cancer via down-regulating CD47. Bioscience Reports, 40(4), BSR20192761.
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3

Apoptosis Quantification by Flow Cytometry

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Cell death was detected using an annexin V-fluorescein isothiocyanate (FITC) and propidium iodide (PI) Apoptosis Detection Kit (Dojindo, China) according to the manufacturer’s instructions. After different treatments, adherent and floating cells were collected, washed twice with PBS, and incubated in annexin V and PI for 15 min at room temperature in the dark. At least 1.0 × 104 cells were collected and detected with a flow cytometer (FACSVerse, Bioscience, USA), and the data were analysed by FlowJo software.
Zhang Y., Zhang J., Zhao Y., Zhang Y., Liu L., Xu X., Wang X, & Fu J. (2023). ChemR23 activation attenuates cognitive impairment in chronic cerebral hypoperfusion by inhibiting NLRP3 inflammasome-induced neuronal pyroptosis. Cell Death & Disease, 14(11), 721.
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4

Quantifying Apoptosis via Annexin V-FITC

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After cells were harvested and washed with cold PBS. The Annexin V‐fluorescein isothiocyanate (FITC) and Propidium Iodide (PI) apoptosis detection kit (Dojindo) was applied to quantitatively estimate the level of phosphatidylserine on cell surface according to the manufacturer's instruction. A flow cytometer (Beckman) was used to analyze the apoptotic cells. A minimum of 10 000 events was collected for each sample. The percentage of apoptotic cells to total cells was shown as the results.
Sun M., Li Z., Wang X., Zhao M., Chu Y., Zhang Z., Fang K., Zhao Z., Feng A., Leng Z., Shi J., Zhang L., Chen T, & Xu M. (2023). TAOK3 Facilitates Esophageal Squamous Cell Carcinoma Progression and Cisplatin Resistance Through Augmenting Autophagy Mediated by IRGM. Advanced Science, 10(29), 2300864.
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