Human mature vegf c

Manufactured by R&D Systems

Human mature VEGF-C is a recombinant protein that corresponds to the mature form of the vascular endothelial growth factor C (VEGF-C) protein. VEGF-C is a member of the VEGF family and is involved in the regulation of angiogenesis and lymphangiogenesis.

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Lab products found in correlation

Purcol, by Advanced BioMatrix (2 mentions) Anti vegfr2, by Cell Signaling Technology (2 mentions) Duolink assay, by Olink (2 mentions)

Close spelling variants of this product

VEGF-C Human VEGF

2 protocols using human mature vegf c

VEGFR-3 and Syndecan Interactions in Lymphangiogenesis

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Chamber slides (Lab-Tek) coated with 50-μg/mL PurCol (Advanced Biomatrix), layered with serum-starved hLEC were stimulated with human mature VEGF-C (R&D) for 5 minutes ± pre treatment with heparinase in some experiments. Cells were fixed in ice-cold methanol for 10 minutes and blocked (Olink PLA-blocking reagent; 30 minutes at 37°C). Rabbit antihuman VEGFR-3 (Reliatech; or for some studies, anti–VEGFR-2; Cell Signaling) and goat antihuman Sdc-4 (R&D) were then added (2 µg/mL) together (in blocking solution overnight; 4°C). After wash, Rabbit(−) and Goat(+) PLA Probes (Duolink assay; Olink Bioscience) were added (1:10 dilution). In other experiments, rabbit anti–VEGFR-3 was paired with either mouse antihuman Sdc-1 (Abcam) or mouse antihuman Sdc-2 (kind gift from G. David) antibodies. Antibodies were directed against extracellular domains, as per manufacturer protocol (imaging: 40× objective, room temperature).

Johns S.C., Yin X., Jeltsch M., Bishop J.R., Schuksz M., El Ghazal R., Wilcox-Adelman S.A., Alitalo K, & Fuster M.M. (2016). Functional Importance of a Proteoglycan Coreceptor in Pathologic Lymphangiogenesis. Circulation Research, 119(2), 210-221.

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VEGFR-3 and Syndecan Interaction Analysis

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Chamber slides (Lab-Tek) coated with 50μg/mL PurCol (Advanced Biomatrix), layered with serum-starved hLEC were stimulated with human mature VEGF-C (R&D) for 5min +/−pre-treatment with heparinase in some experiments. Cells were fixed in ice-cold methanol for 10min and blocked (Olink PLA-blocking reagent; 30min at 37°C). Rabbit-anti-human VEGFR-3 (Reliatech) (or for some studies, anti-VEGFR-2; Cell Signaling) and goat-anti-human syndecan-4 (R&D) were then added (2μg/ml) together (in blocking solution overnight; 4°C). Following wash, Rabbit(−) and Goat(+) PLA Probes (Duolink assay; Olink Bioscience) were added (1:10 dilution). In other experiments, rabbit-anti-VEGFR-3 was paired with either mouse-anti-human syndecan-1 (Abcam) or mouse-anti-human syndecan-2 (kind gift from G. David) antibodies. Antibodies were directed against extracellular domains, with manufacturer protocol followed (imaging: 40× objective, RT).

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