Log In Sign up
The largest database of trusted experimental protocols

Ef2 sc 13004 r

Manufactured by Santa Cruz Biotechnology
Visit Supplier

The EF2 (sc-13004-R) is a research-use laboratory equipment product offered by Santa Cruz Biotechnology. It serves as a core component for various scientific applications. The detailed specifications and intended uses of this product are not available for an unbiased and factual presentation.

Automatically generated - may contain errors

Lab products found in correlation

Alexa fluor 647 conjugated phalloidin, by Thermo Fisher Scientific (2 mentions) α tubulin, by Merck Group (2 mentions) Sept9, by OriGene (2 mentions) Sept6, by Santa Cruz Biotechnology (2 mentions) Sept8, by Santa Cruz Biotechnology (2 mentions) Gapdh, by Merck Group (2 mentions) Anti goat secondary antibody, by Santa Cruz Biotechnology (1 mentions) Alexa dye labeled secondary antibodies, by Thermo Fisher Scientific (1 mentions) P38 mapk, by Cell Signaling Technology (1 mentions) Interleukin 3 (il 3), by Thermo Fisher Scientific (1 mentions) Stem cell factor (scf), by Thermo Fisher Scientific (1 mentions) Phospho histone h3, by Cell Signaling Technology (1 mentions) Stathmin, by Cell Signaling Technology (1 mentions) Propidium iodide p4170, by Merck Group (1 mentions) Acetyl tubulin, by Merck Group (1 mentions) Cleaved caspase 3, by Cell Signaling Technology (1 mentions) Interleukin 6 (il 6), by Thermo Fisher Scientific (1 mentions) Interleukin 2 (il 2), by Immunotools (1 mentions)

2 protocols using ef2 sc 13004 r

1

Antibody Reagents for Septin-Based Research

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Anti-SEPT7 antibodies were obtained from IBL international (#JP18991), rabbit anti-anillin antibodies were reported earlier40 (link). Other antibodies used were SEPT2 (#11397-1-AP, Acris), SEPT9 (#10769-1-AP, Acris), SEPT6 (sc-20180, Santa Cruz Biotech), SEPT8 (sc-48937, Santa Cruz Biotech), EF2 (sc-13004-R, Santa Cruz Biotech), GAPDH (#MAB374, Millipore), tubulin-α (T6199, Sigma), GFP (sc-9996, Santa Cruz Biotech), GST (sc-138, Santa Cruz Biotech), FLAG (#200472-21, Stratagene) and p38 MAPK (#9212, Cell Signaling Technology). All Alexa-dye labeled secondary antibodies and Alexa fluor-647-conjugated phalloidin (#A22287) were from Invitrogen. HRP-labeled anti-mouse (#115-035-003) and anti-rabbit (#111-035-003) secondary antibodies were from Dianova; anti-goat secondary antibody was from Santa Cruz Biotech (sc-2033). DAPI for DNA staining was from Carl Roth (#6335.1). Polybrene (H9268), doxycycline (D9891) were from Sigma.
Abbey M., Hakim C., Anand R., Lafera J., Schambach A., Kispert A., Taft M.H., Kaever V., Kotlyarov A., Gaestel M, & Menon M.B. (2016). GTPase domain driven dimerization of SEPT7 is dispensable for the critical role of septins in fibroblast cytokinesis. Scientific Reports, 6, 20007.
+ Open protocol
+ Expand
2

Antibody Staining Reagents and Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibody against SEPT7 was from IBL international (#JP18991), Rabbit anti-anillin antibodies were reported earlier (Watanabe et al., 2010). Antibodies used for western blot analysis were SEPT2 (#11397-1-AP, Acris), SEPT9 (#10769-1-AP, Acris), SEPT6 (sc-20180, Santa Cruz Biotech), SEPT8 (sc-48937, Santa Cruz Biotech), EF2 (sc-13004-R, Santa Cruz Biotech), GAPDH (#MAB374, Millipore), GFP (sc-9996, Santa Cruz Biotech) and Stathmin (#3352, Cell Signaling Technology). Antibodies used for Immunofluorescence staining were rabbit anti SEPT7, SEPT2, SEPT6 [23] (link), Ki67, phospho-Histone-H3, cleaved caspase-3 (Cell Signaling Technology), tubulin-α (T6199, Sigma and sc-31779, Santa Cruz Biotech), LAP2 (#611000, BD Transduction lab) and acetyl tubulin (T6793, Sigma). All alexa-dye labeled secondary antibodies, tetramethyl rhodamine-conjugated WGA (#W849) and Alexa fluor-647-conjugated phalloidin (#A22287) were from Invitrogen. DAPI for DNA staining was from Carl Roth (#6335.1). Polybrene (H9268), doxycycline (D9891), RNAse A (R4875) and propidium iodide (P4170) were from Sigma. Forchlorfenuron (FCF) was obtained from Santa Cruz Biotech. IL2 was from ImmunoTools. IL3, IL6 and SCF were from Peprotech.
Menon M.B., Sawada A., Chaturvedi A., Mishra P., Schuster-Gossler K., Galla M., Schambach A., Gossler A., Förster R., Heuser M., Kotlyarov A., Kinoshita M, & Gaestel M. (2014). Genetic Deletion of SEPT7 Reveals a Cell Type-Specific Role of Septins in Microtubule Destabilization for the Completion of Cytokinesis. PLoS Genetics, 10(8), e1004558.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!