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Pe conjugated mouse anti chicken cd3 antibody

Manufactured by Southern Biotech
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Sourced in United States

The PE)-conjugated mouse anti-chicken CD3 antibody is a laboratory reagent used for the detection and analysis of chicken T cells. It binds specifically to the CD3 antigen expressed on the surface of chicken T lymphocytes. The PE (Phycoerythrin) fluorescent label allows for the identification and quantification of CD3-positive cells using flow cytometry or other immunoassay techniques.

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Lab products found in correlation

Fitc conjugated mouse anti chicken cd4 antibody, by Southern Biotech (2 mentions) Fitc conjugated mouse anti chicken cd8α antibody, by Southern Biotech (2 mentions) Facsc 2000, by BD (2 mentions)

Close spelling variants of this product

Mouse anti-chicken CD3: PE (2 citations) CD3 PE (2 citations) Anti-chicken CD3 mouse antibody (1 citations)

2 protocols using pe conjugated mouse anti chicken cd3 antibody

1

Chicken Antibody and T-cell Response Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
At 0 and 5 dpi, the serum antibodies of chickens were detected by enzyme-linked immunosorbent assay (ELISA) as described previously (56 (link)). All samples were analyzed in triplicate.
To measure the percentages of CD4+ and CD8+ cells among CD3+ cells, peripheral blood lymphocytes (PBLs) were incubated with phycoerythrin (PE)-conjugated mouse anti-chicken CD3 antibody (Southern Biotech, USA) and fluorescein isothiocyanate (FITC)-conjugated mouse anti-chicken CD4 antibody (Southern Biotech) or FITC-conjugated mouse anti-chicken CD8α antibody (Southern Biotech) at 4°C for 1 h. After being washed with PBS, the PBLs were analyzed by fluorescence-activated cell sorter (FACS; FACSC 2000, BD).
Zhao N., Ming S., Sun L., Wang B., Li H., Zhang X, & Zhao X. (2021). Identification and Characterization of Eimeria tenella Microneme Protein (EtMIC8). Microbiology Spectrum, 9(1), 10.1128/spectrum.00228-21.
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2

Chicken Antibody and T-cell Response Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
At 0 and 5 dpi, the serum antibodies of chickens were detected by enzyme-linked immunosorbent assay (ELISA) as described previously (56 (link)). All samples were analyzed in triplicate.
To measure the percentages of CD4+ and CD8+ cells among CD3+ cells, peripheral blood lymphocytes (PBLs) were incubated with phycoerythrin (PE)-conjugated mouse anti-chicken CD3 antibody (Southern Biotech, USA) and fluorescein isothiocyanate (FITC)-conjugated mouse anti-chicken CD4 antibody (Southern Biotech) or FITC-conjugated mouse anti-chicken CD8α antibody (Southern Biotech) at 4°C for 1 h. After being washed with PBS, the PBLs were analyzed by fluorescence-activated cell sorter (FACS; FACSC 2000, BD).
Zhao N., Ming S., Sun L., Wang B., Li H., Zhang X, & Zhao X. (2021). Identification and Characterization of Eimeria tenella Microneme Protein (EtMIC8). Microbiology Spectrum, 9(1), 10.1128/spectrum.00228-21.
+ Open protocol
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