Ethanol, acetone, 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), Folin–Ciocalteu reagent, KCl, NaHCO3, KH2PO4, (NH4)2CO3, NaCl, HCl, MgCl2·(H2O)6 and thin-layer chromatography (TLC) plates were purchased from Merck (Darmstadt, Germany). α-amylase, pepsin from porcine gastric mucosa, pancreatin from porcine pancreas and bile salts were purchased from Alfa Aesar (Karslruhe, Germany). All reagents used in the experimental part were of analytical purity.
α amylase
Manufactured by Thermo Fisher Scientific
Sourced in Germany
α-amylase is an enzyme that catalyzes the hydrolysis of starch into smaller carbohydrate molecules. It functions by breaking down the α-1,4 glycosidic bonds in starch, converting it into maltose and other smaller oligosaccharides.
Automatically generated - may contain errors
Lab products found in correlation
Pancreatin from porcine pancreas, by Thermo Fisher Scientific (2 mentions)
Pepsin from porcine gastric mucosa, by Thermo Fisher Scientific (2 mentions)
Bile salt, by Thermo Fisher Scientific (2 mentions)
Mgcl2 h2o 6, by Merck Group (2 mentions)
Nh4 2co3, by Merck Group (2 mentions)
Folin ciocalteu reagent, by Merck Group (2 mentions)
Sodium chloride (nacl), by Merck Group (2 mentions)
Potassium chloride (kcl), by Merck Group (2 mentions)
Kh2po4, by Merck Group (2 mentions)
Nahco3, by Merck Group (2 mentions)
Hydrochloric acid (hcl), by Merck Group (2 mentions)
Thin layer chromatography, by Merck Group (1 mentions)
2 2 azinobis 3, by Merck Group (1 mentions)
Ethanol, by Merck Group (1 mentions)
Acetone, by Merck Group (1 mentions)
H2so4 0 1n, by Merck Group (1 mentions)
Ethanol 96, by Merck Group (1 mentions)
Ascorbic acid, by Merck Group (1 mentions)
Kmno4, by Merck Group (1 mentions)
Glacial acetic acid, by Merck Group (1 mentions)
2 protocols using α amylase
1
In Vitro Bioaccessibility Analysis
2
Comprehensive Analytical Characterization of Sweeteners
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Ethanol 96%, 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 2,4,6-tripyridyl-s-triazine (TPTZ), FeCl3·6H2O,Folin–Ciocalteu reagent, ascorbic acid, CH3COONa, 36.5% HCl, glacial acetic acid, H2SO4 (0.1 N), KMnO4, KCl, NaHCO3, KH2PO4, (NH4)2CO3, NaCl, MgCl2⋦(H2O)6 were purchased from Merck. α-Amylase, pepsin from porcine gastric mucosa, pancreatin from porcine pancreas, bile salts were purchased from Alfa Aesar (Karslruhe, Germany). All reagents used in the experimental part were of analytical purity. Sweeteners used in this study were purchased from a health food store and local market.
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