Plan apo 10 1

Images were acquired using an epifluorescence microscope (Nikon Eclipse 90i, Nikon) equipped with a × 10 objective (Plan Apo × 10/1, Nikon) or a confocal (Nikon A1R, Nikon) microscope equipped with dry × 10 and × 20 objectives (CFI Plan Apo × 10/0.45 and CFI Plan Apo VC; × 20/0.75, Nikon). Laminar distribution of IN subtypes was quantified on both hemispheres of three coronal sections of brains from SERT-ko, PF-exposed mice and pellet control mice using equally spaced bins apposed at the level of the primary somatosensory cortex. Bins corresponding to cortical layers 1 to 6 were pooled based on cytological differences in the density of Hoechst staining.

Images were acquired using an epifluorescence microscope (Nikon, Eclipse 90i) equipped with a × 10 objective (Plan Apo × 10/1, Nikon) or a confocal (Nikon, A1R) microscope equipped with dry × 10 and × 20 objectives (CFI Plan Apo × 10/0.45 and CFI Plan Apo VC; × 20/0.75, Nikon) and oil-immersion × 40 and × 60 objectives (CFI Plan Fluor × 40/1.3 and CFI Plan Apo VC H × 60/1.4, Nikon). Quantification of the distribution of IN subtypes in the cortex was done by apposing a 10-bin grid at the level of the primary somatosensory cortex and bins corresponding to cortical layers were pooled.