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Efluor660 anti il 21

Manufactured by Thermo Fisher Scientific
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EFluor660-anti–IL-21 is a fluorescently-labeled antibody that binds to the cytokine interleukin-21 (IL-21). It is designed for use in flow cytometry and other immunoassays to detect and quantify IL-21 expression.

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Lab products found in correlation

Anti cd45ra fitc, by Thermo Fisher Scientific (3 mentions) Percp cy5.5 anti ifn γ, by Thermo Fisher Scientific (2 mentions) Pacificblue anti cd20, by BioLegend (2 mentions) Biotin pd 1, by Thermo Fisher Scientific (2 mentions) Sa bv605, by BioLegend (2 mentions) Recombinant human il 12, by R&D Systems (2 mentions) Anti cd4 apc cy7, by BD (2 mentions) Anti cd25 pe cy7, by BD (2 mentions) Anti icos pe, by Thermo Fisher Scientific (2 mentions) Sa percpcy5, by BD (2 mentions) Bcl 6, by BD (1 mentions) Il 23, by Thermo Fisher Scientific (1 mentions) Anti pstat3, by BD (1 mentions) Anti t bet, by Thermo Fisher Scientific (1 mentions) Alexa fluor 647 anti cxcr5, by BD (1 mentions) Anti t bet, by BD (1 mentions) Alexa fluor 488 anti il 10, by Thermo Fisher Scientific (1 mentions) Anti p stat4, by BD (1 mentions) Anti pstat5, by BD (1 mentions) Anti cd27, by BD (1 mentions)

4 protocols using efluor660 anti il 21

1

Multiparametric Profiling of T Cell Subsets

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eFluor660-anti–IL-21, Alexa Fluor 488–anti–IL-10, PerCP-Cy5.5-anti–IFN-γ, FITC-anti-CD45RA, PE-anti-ICOS, and anti-Tbet, and biotin–PD-1 were from eBioscience. Alexa Fluor 488–anti-GATA3, Alexa Fluor 647–anti-CXCR5, anti-pSTAT4, anti-pSTAT5, anti-pSTAT6, APC-anti-CD10, APC-Cy7-anti-CD4, BV605-anti-IgG, BV421-CD40L, BV711-anti–IL-2, PE-anti-pSTAT1, anti-RORγt, and Bcl-6, PE-Cy7-anti-CD25, and anti-CD27, PerCP-Cy5.5-anti-CD127, anti-pSTAT3, and anti-Tbet, SA-PerCpCy5.5, and IFN-γ were obtained from BD. Pacific Blue–anti-CD20 and SA-BV605 were purchased from BioLegend. Recombinant human IL-12 was purchased from R&D Systems. TGF-β, IL-1β, IL-6, IL-21, and IL-23 were obtained from PeproTech. PGE2 was purchased from Sigma-Aldrich. Human IL-4 and IL-10 were provided by R. de Waal Malefyt (DNAX Research Institute, Palo Alto, CA).
Ma C.S., Wong N., Rao G., Nguyen A., Avery D.T., Payne K., Torpy J., O’Young P., Deenick E., Bustamante J., Puel A., Okada S., Kobayashi M., Martinez-Barricarte R., Elliott M., Sebnem Kilic S., El Baghdadi J., Minegishi Y., Bousfiha A., Robertson N., Hambleton S., Arkwright P.D., French M., Blincoe A.K., Hsu P., Campbell D.E., Stormon M.O., Wong M., Adelstein S., Fulcher D.A., Cook M.C., Stepensky P., Boztug K., Beier R., Ikincioğullari A., Ziegler J.B., Gray P., Picard C., Boisson-Dupuis S., Phan T.G., Grimbacher B., Warnatz K., Holland S.M., Uzel G., Casanova J.L, & Tangye S.G. (2016). Unique and shared signaling pathways cooperate to regulate the differentiation of human CD4+ T cells into distinct effector subsets. The Journal of Experimental Medicine, 213(8), 1589-1608.
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2

Multi-Parameter Flow Cytometry Protocol

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eFluor660-anti-IL-21, PerCP-Cy5.5-anti-IFNγ, FITC-anti-CD45RA, biotin-PD-1 were from eBiosciences. Alexa647-anti-CXCR5 and anti-pSTAT1, APC-anti-CD10, APC-Cy7-anti-CD4, BV605-anti-IgG, PE-anti-pSTAT3 and anti-CCR6, Pe-Cy7-anti-CD25 and anti-CD27, PerCpCy5.5-anti-CD127, biotin-anti-IgA, SA-PerCpCy5.5, and recombinant IFNγ were from Becton Dickinson. BV421-anti-CXCR3, Pacific Blue-anti-CD20 and SA-BV605 were from Biolegend.
Ma C.S., Wong N., Rao G., Avery D.T., Torpy J., Hambridge T., Bustamante J., Okada S., Stoddard J.L., Deenick E.K., Pelham S.J., Payne K., Boisson-Dupuis S., Puel A., Kobayashi M., Arkwright P.D., Kilic S.S., Baghdadi J.E., Nonoyama S., Minegishi Y., Mahdaviani S.A., Mansouri D., Bousfiha A., Blincoe A.K., French M.A., Hsu P., Campbell D.E., Stormon M.O., Wong M., Adelstein S., Smart J.M., Fulcher D.A., Cook M.C., Phan T.G., Stepensky P., Boztug K., Kansu A., Ikincioğullari A., Baumann U., Beier R., Roscioli T., Ziegler J.B., Gray P., Picard C., Grimbacher B., Warnatz K., Holland S.M., Casanova J.L., Uzel G, & Tangye S.G. (2015). Monogenic mutations differentially impact the quantity and quality of T follicular helper cells in human primary immunodeficiencies. The Journal of allergy and clinical immunology, 136(4), 993-1006.e1.
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3

Comprehensive Immunophenotyping Panel

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The following were purchased from eBiosciences: anti-IL-21 eFluor660, anti-IL-10 Alexa Fluor 488, anti-IFNγ BV605, anti-CD45RA FITC, anti-ICOS PE, anti-PD-1 biotin. The following were purchased from Becton Dickinson: anti-CXCR5 Alexa Fluor 647, anti-CD4 APC-Cy7, anti-IL-2 BV711, anti-CD25 PE-Cy7, anti-CD127 PerCP-Cy5.5, SA-PerCpCy5.5, anti-IL-17F BV786, anti-TNFα BUV395, anti-IL-9 PerCP-Cy5.5, anti-IL-13 BV421. The following were purchased from Biolegend: anti-IL-22 PE, anti-IL-4 PE-Cy7, anti-IL-17A APC-Cy7. The TCR Vß repertoire kit was from Beckman Coulter. Recombinant human IL-12 was purchased from R&D Systems.
Bier J., Rao G., Payne K., Brigden H., French E., Pelham S.J., Lau A., Lenthall H., Edwards E.S., Smart J.M., Cole T.S., Choo S., Joshi A.Y., Abraham R.S., O’Sullivan M., Boztug K., Meyts I., Gray P.E., Berglund L.J., Hsu P., Wong M., Holland S.M., Notarangelo L.D., Uzel G., Ma C.S., Brink R., Tangye S.G, & Deenick E.K. (2019). Activating mutations in PIK3CD and murine CD4+ T cells. The Journal of allergy and clinical immunology, 144(1), 236-253.
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4

Multiparameter Flow Cytometry of CD8+ T Cell Subsets

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To analyze the frequency of CD8+ T cell subsets, the harvested cells were washed once with PBS and evaluated by flow cytometry. In brief, the 106 cells/100ul staining buffer (PBS + 2%FCS) were first stained with Amcyan-conjugated anti-human CD8 monoclonal antibodies (BD Biosciences, US) for 30 minutes in the dark at 4°c. After surface staining, the cells were fixed and permeabilized using LEUCOPERM™ (BIO-RAD, BUF09B, US) according to the manufacturer’s instructions. The cells were stained with anti-IFN-γ-FITC, anti-TNF-α-PE-Cy™7, anti-IL-10-eFluor®450, anti-IL-17-PE, anti-IL-21-eFluor®660, anti-IL-22-PerCP-eFluor®710 (eBioscience, US) and anti- IL-4-APC-Cy™7 (BioLegend, US).
Salehi Z., Doosti R., Beheshti M., Janzamin E., Sahraian M.A, & Izad M. (2016). Differential Frequency of CD8+ T Cell Subsets in Multiple Sclerosis Patients with Various Clinical Patterns. PLoS ONE, 11(7), e0159565.
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