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Tris hcl

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Tris-HCl is a buffering agent commonly used in various laboratory applications. It is a salt formed by the combination of tris(hydroxymethyl)aminomethane (Tris) and hydrochloric acid (HCl). Tris-HCl is used to maintain a specific pH range in buffer solutions, facilitating the control of the chemical environment in experiments and assays.

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Lab products found in correlation

Glycine, by Vivantis Technologies (2 mentions) 0.45 m membrane filter, by Pall Corporation (1 mentions) Ampicillin sodium salt, by Merck Group (1 mentions) Corn starch, by Merck Group (1 mentions) Isopropyl β d 1 thiogalactopyranoside (iptg), by Vivantis Technologies (1 mentions) Tlc silica gel 60 f254 glass plates, by Merck Group (1 mentions) Aquacide 2, by Merck Group (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Isopropanol, by Merck Group (1 mentions) Methanol, by Merck Group (1 mentions) Sulfuric acid, by Carlo Erba (1 mentions) Hematoxylin eosin, by Merck Group (1 mentions) Xylol, by Merck Group (1 mentions) Ammonium sulfate, by Merck Group (1 mentions) Paraffin, by Merck Group (1 mentions) Agarose, by HiMedia (1 mentions) Absolute ethanol, by Merck Group (1 mentions)

3 protocols using tris hcl

1

Purification of Plant-Produced Antibody Fragments

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Infiltrated N. benthamiana leaves were extracted with 1X PBS, pH 7.4 using a blender (JSR, Gongju-si, Korea) and centrifuged (Thermo Scientific, Massachusetts, USA) at 26,000 g for 30 min. The supernatant was filtered with a 0.45 µm membrane filter (Pall Corporation, New York, USA) and loaded onto a protein-A affinity column. The column was washed with ten-column volumes of PBS pH 7.4. The bound protein was eluted with 0.1 M glycine (Vivantis, Shah Alam, Malaysia), pH 2.7, and rapidly neutralized to pH 7.4 with 1.5 M Tris–HCl (Vivantis, Shah Alam, Malaysia), pH 8.8. The purified plant-produced VHH-αNTX-Fc and VHH-PLA2-Fc antibody was analyzed using SDS-PAGE and immunoblot analysis.
Vitayathikornnasak S., Rattanapisit K., Malla A., Suwanchaikasem P., Strasser R., Khorattanakulchai N., Pothisamutyothin K., Arunmanee W, & Phoolcharoen W. (2024). Characterization of plant produced VHH antibodies against cobra venom toxins for antivenom therapy. Biotechnology Reports, 42, e00841.
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2

Carbohydrate-based Analytical Procedures

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Maltooligosaccharide (G1-4), soluble potato starch, corn starch, bovine serum albumin and ampicillin sodium salt were purchased from Sigma-Aldrich (St. Louis, MO, USA). Tryptone and yeast extract were obtained from Difco (Bacton Dickinson and company, Sparks, MD, USA). β-CD was purchased from AppliChem (Darmstadt, Germany). Standard maltitol and D-sorbitol were obtained from TCI (Toshima, Kita-ku, Tokyo, Japan). Aquacide II, methanol, isopropanol, ethyl acetate, acetonitrile, HPLC-grade water, potassium dihydrogen phosphate, dipotassium phosphate and TLC silica gel 60 F254 glass plates (20 cm in height) were purchased from Merck (Darmstadt, Germany). IPTG, glycine and Tris-HCl were obtained from Vivantis (Sendirian Berhad, Shah Alam, Malaysia). Sulfuric acid was obtained from Carlo Erba Reagents (Val de Reuil, France). The commercial glucose oxidase kit was obtained from Human mit-diagnostics GmbH (Idstein, Germany). Other chemicals used were of an analytical grade.
Haewpetch P., Rudeekulthamrong P, & Kaulpiboon J. (2022). Enzymatic Synthesis of Maltitol and Its Inhibitory Effect on the Growth of Streptococcus mutans DMST 18777. Biomolecules, 12(2), 167.
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3

Biochemical Analysis of Experimental Samples

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Materials used in this study were ammonium sulfate (Merck, Denmark), agarose (Himedia, India), de Man Rogosa Sharpe Broth (Himedia, India), Luria Bertani (Himedia, India), Hematoxylin-Eosin, formalin 4%, ethanol absolute (Merck, Denmark), xylol, paraffin, glycerin 99.5%, NaCl (Merck, Denmark), and TCA, TBA, BHT, Tris-HCl (Vivantis, USA).
Ahaddin A.Y., Budiarti S., Mustopa A.Z., Darusman H.S., & Triratna L. (2020). Short Communication: Acute toxicity study of plantaricin from Lactobacillus plantarum S34 and its antibacterial activity. Biodiversitas Journal of Biological Diversity, 22(1).
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