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Special grade reagent

Manufactured by Fujifilm
Sourced in Japan

Special-grade reagent is a high-purity chemical compound designed for use in laboratory settings. It is formulated to meet the rigorous standards and specifications required for precision analytical and research applications.

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Lab products found in correlation

2 protocols using special grade reagent

1

Solvent-Based Silica Particle Characterization

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A special-grade reagent manufactured by Wako Pure Chemical Industries, Ltd. was used as the organic solvent, and Hypressic N2N spherical silica particles (Ube-exsymo Ltd. Tokyo, Japan) were used as model particles. A list of solvents used in this study is shown in Table S1. The primary particle diameter of the silica particles was 500 nm. In addition, in this study, the solvent set used for measuring the HSP and light transmittance differed. The solvent set used for the Hansen dissolving sphere method should be present in a wide area in Hansen's 3D graph. Experiments were conducted focusing on the value of δd in the light transmittance measurement. Therefore, the solvent set used for determining the light transmittance was selected such that the value of δd was widely dispersed in the range of 14.5–21.0 (MPa)1/2.
A total of three polymers were used as solid-solid dispersion media. Two types of polymers with different styrene-methacrylic acid complex ratios and Silicone-methacrylic acid composite adhesive. Because CEMEDINE C is a solvent-based adhesive, it was necessary to remove the solvent. To obtain only the polymer part, the adhesive was placed on a Teflon sheet in air for 24 h to sufficiently remove the solvent, and the polymer part was used in the experiment. In this paper, the polymer part from which the solvent has been removed is called CEMEDINE C.
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2

Amino Acid Profiling of BR and SBR

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The contents of 24 amino acids in BR and SBR were measured.
The extraction procedure for amino acids followed that reported by Morita (2017) . The dried grains, buds, and roots of BR and SBR after weighing were collected and crushed with a mill (Silent Millser IFM-S30G; Iwatani, Japan). A powdered sample (1.0 g) was mixed with 9 mL of 2 % sulfosalicylic acid solution (Special grade reagent; FUJIFILM Wako Pure Chemical Corporation, Japan) in a 50-mL plastic conical tube, and the mixture was shaken in a warm bath at 25 ˚C for 30 min.
The mixture was centrifuged at 5 500 rpm for 10 min and the supernatant was filtered through a 0.45-µm filter to obtain the extract.
Amino acids were analysed using an automatic amino acid analyser (JLC-500/V2; JEOL Ltd., Japan). The postcolumn ninhydrin method was used with 50 µL of a sample in the high-resolution mode for free amino acids in lithium citrate buffer. The content of each amino acid was determined by comparing the peak area of the obtained sample with the peak area of the standard sample (amino acid mixed standard solution, AN-2 type and B type in equal amounts; FUJIFILM Wako Pure Chemical Corporation, Japan).
One measurement was carried out for each independent experiment, and the results of three measurements are shown as the average value per 100 g dw ± standard deviation.
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