Monoclonal anti type 1 and 4 collagens

Phosphate-buffered saline (PBS) and Dulbecco’s modified eagle medium (DMEM) were purchased from Welgene (Daegu, Republic of Korea) and the EZ-CyTox kit was from DAEIL LAB Service (Seoul, Republic of Korea). FBS, penicillin/streptomycin (P/S), and trypsin-ethylenediamine tetra-acetic acid (EDTA) were purchased from Gibco BRL (Gaithersburg, MD, USA). Dimethyl sulfoxide (DMSO) was purchased from MilliporeSigma (St. Louis, MO, USA), and recombinant keratinocyte growth factor (KGF), recombinant human EGF (purity > 97%), and tumor necrosis factor-α (TNF-α) were from R&D Systems (Minneapolis, MN, USA). Bovine serum albumin (BSA) and type I collagen were obtained from BD Bioscience (Franklin Lakes, NJ, USA). The antibodies used were anti-rabbit IgG, anti-mouse IgG, anti-AKT, anti-phospho AKT, anti-JNK, anti-phospho JNK, anti-p38 MAPK, anti-phospho p38 MAPK, anti-ERK1/2, anti-phospho ERK1/2 (Cell Signaling, Beverly, MA, USA), anti-HAS-1, anti-HAS-2, anti-HAS-3 (Novus Biologicals, Littleton, CO, USA), polyclonal anti-type I and IV collagens, monoclonal anti-type I and IV collagens (Abcam, Cambridge, UK), and β-actin (MilliporeSigma, Burlington, MA, USA).

Trypsin-ethylenediamine tetra-acetic acid (EDTA), FBS, and penicillin/streptomycin (P/S) were purchased from Gibco BRL (Gaithersburg, MD, USA), and phosphate buffered saline (PBS) and Dulbecco’s modified eagle medium (DMEM) were from Welgene (Daegu, South Korea). Bovine serum albumin (BSA), L-DOPA, Triton X-100, phenylmethylsulfonyl fluoride, α-MSH, and dimethyl sulfoxide (DMSO) were purchased from MilliporeSigma (St. Louis, MO, USA), and recombinant human EGF (purity > 97%) was from R&D Systems (Minneapolis, MN, USA). The EZ-CyTox kit was purchased from DoGenBio (Seoul, Korea) and type I collagen from BD Bioscience (Franklin Lakes, NJ, USA). The antibodies used were anti-ERK1/2, anti-phospho ERK1/2, anti-p38 MAPK, anti-phospho p38 MAPK, anti-JNK, anti-phospho JNK, anti-AKT, anti-phospho AKT, anti-MITF, anti-rabbit IgG, and anti-mouse IgG (Cell Signaling, Beverly, MA, USA), monoclonal anti-type I and IV collagens, polyclonal anti-type I and IV collagen (Abcam), anti-tyrosinase (Santa Cruz Biotechnology, CA, USA), and β-actin (MilliporeSigma, St. Louis, MO, USA).