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Arachidonic acid

Manufactured by Fujifilm
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Sourced in Japan

Arachidonic acid is a polyunsaturated fatty acid found in cell membranes. It serves as a precursor for the synthesis of various eicosanoids, which are signaling molecules involved in inflammatory and other physiological processes.

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Lab products found in correlation

Capric acid, by Fujifilm (1 mentions) Myristic acid, by Fujifilm (1 mentions) Stearic acid, by Fujifilm (1 mentions) Lauric acid, by Fujifilm (1 mentions) Caprylic acid, by Fujifilm (1 mentions)

2 protocols using arachidonic acid

1

Fatty Acid and Lipid Standards

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Caprylic acid (C8:0), capric acid (C10:0), lauric acid (C12:0), myristic acid (C14:0), stearic acid (C18:0), arachidonic acid (C20:4n6), and methyl-d3 stearate standard were obtained from Wako Pure Chemicals (Tokyo, Japan).
Arita K., Honma T, & Suzuki S. (2017). Comprehensive and comparative lipidome analysis of Vitis vinifera L. cv. Pinot Noir and Japanese indigenous V. vinifera L. cv. Koshu grape berries. PLoS ONE, 12(10), e0186952.
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2

Measuring PGE2 Synthesis Enzymatic Activity

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Enzymatic activity related to PGE2 synthesis was determined by measuring the accumulation of PGE2 induced by arachidonic acid (AA; Wako Chemical) in the culture fluids. Briefly, Het-1A cells (5.0 × 104/well) and KYSE-270 cells (2.5 × 104/well) were cultured overnight in 96-well plates. The culture fluids were replaced with the same fresh medium containing HST or the COX-2 inhibitor NS-398 for 15 min, and AA was added to the culture medium at a final concentration of 3 μmol/L. PGE2 concentrations were measured as described above after further incubation for 15 min.
Sadatomi D., Kono T., Mogami S, & Fujitsuka N. (2020). Weak acids induce PGE2 production in human oesophageal cells: novel mechanisms underlying GERD symptoms. Scientific Reports, 10, 20775.
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