An ELISA for total serum IgE was performed according to the manufacturer’s instructions using a Mouse IgE ELISA kit (#88-50460-88; Invitrogen); serum was applied at a concentration of 1:50. To measure peanut-specific IgE, a clear flat-bottom immuno nonsterile 96-well plate (#442404; Thermo Fisher Scientific) was coated with peanut extract in PBS (10 µg/ml) instead of the anti-IgE capture antibody, and the rest of the assay was performed using Mouse IgE ELISA kit (#88-50460-88; Invitrogen) according to the manufacturer’s instructions; serum was applied at a concentration of 1:50. To measure peanut-specific IgG1, a clear flat-bottom immuno nonsterile 96-well plate (#442404; Thermo Fisher Scientific) was coated with peanut extract in PBS (10 µg/ml) instead of the anti-IgG1 capture antibody, the rest of the assay was performed using a Mouse IgG1 ELISA kit (#88-50410-88; Invitrogen), according to the manufacturer’s instructions; serum was applied at 1:5,000. Serum MCPT-1 levels were measured using an MCPT-1 (mMCP-1) Mouse ELISA Kit (#88-7503-88; Invitrogen) according to the manufacturer’s instructions; serum was applied at a concentration of 1:50.
Mouse igg1 elisa kit
Manufactured by Thermo Fisher Scientific
The Mouse IgG1 ELISA kit is a laboratory assay used to detect and quantify the levels of mouse immunoglobulin G1 (IgG1) in biological samples. The kit utilizes the enzyme-linked immunosorbent assay (ELISA) technique to measure the concentration of mouse IgG1 in the sample.
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Lab products found in correlation
2 protocols using mouse igg1 elisa kit
1
Quantification of Serum Allergic Markers
2
Quantifying Serum Immune Markers in Mice
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An ELISA for total serum IgE was performed according to the manufacturer’s instructions using a Mouse IgE ELISA kit (#88-50460-88; Invitrogen); serum was applied at a concentration of 1:50. To measure peanut-specific IgE, a clear flat-bottom immuno nonsterile 96-well plate (#442404; Thermo Fisher Scientific) was coated with peanut extract in PBS (10 µg/ml) instead of the anti-IgE capture antibody, the rest of the assay was performed using Mouse IgE ELISA kit (#88-50460-88; Invitrogen) according to the manufacturer’s instructions; serum was applied at a concentration of 1:50. To measure peanut-specific IgG1, a clear flat-bottom immuno nonsterile 96-well plate (#442404; Thermo Fisher Scientific) was coated with peanut extract in PBS (10 µg/ml) instead of the anti-IgG1 capture antibody and the rest of the assay was performed using a Mouse IgG1 ELISA kit (#88-50410-88; Invitrogen), according to the manufacturer’s instructions; serum was applied at 1:5,000. Serum MCPT-1 levels were measured using an MCPT-1 (mMCP-1) Mouse ELISA Kit (#88-7503-88; Invitrogen) according to the manufacturer’s instructions; serum was applied at a concentration of 1:50.
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