Cells were stained in the presence of 1:500 Fc block (CD16/32, Tonbo) and antibodies diluted 1:200 in FACs buffer (PBS+2.5% FBS) for 45 minutes in the dark at 4°C. Ghost viability dye BV540 (Tonbo) was used to exclude dead cells at 1:500. Cells were fixed in 2% PFA or fixation buffer (Tonbo) for 10–15 at room temperature in the dark prior to data acquisition. Cells were acquired within 24 h using a Cytek Aurora.
Cd16 32
Manufactured by Cytek Biosciences
Sourced in United States
The CD16/32 is a lab equipment product offered by Cytek Biosciences. It is a receptor that binds to the Fc region of immunoglobulins, facilitating the detection and analysis of various cell types and their interactions. The core function of the CD16/32 is to enable the study of immune cell activation and signaling pathways.
Automatically generated - may contain errors
3 protocols using cd16 32
1
Multiparameter Flow Cytometry Staining
2
Multiparameter Flow Cytometry Staining
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were stained in the presence of 1:500 Fc block (CD16/32, Tonbo) and antibodies diluted 1:200 in FACs buffer (PBS+2.5% FBS) for 45 minutes in the dark at 4°C. Ghost viability dye BV540 (Tonbo) was used to exclude dead cells at 1:500. Cells were fixed in 2% PFA or fixation buffer (Tonbo) for 10–15 at room temperature in the dark prior to data acquisition. Cells were acquired within 24 h using a Cytek Aurora.
3
Analyzing Lung Immune Responses to E-Cigarette Exposure
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mice were sacrificed 24 h after the last e-cigarette exposure and were euthanized by administering a mixture of ketamine and xylazine. Lungs were lavaged via tracheal catheterization three times each with 0.6 mL of 0.05% fetal bovine serum in 0.9% NaCl. The combined lavage fluids were centrifuged at 2000 rpm for 10 min at 4 °C. The supernatant was recovered and stored at −80 °C, while the cell pellet was resuspended in 1 mL of 1 × phosphate buffer saline (PBS). Total cell counts were measured by staining cells with acridine orange and propidium iodide (AO/PI) and counted using the Nexcelom Cellometer Auto 2000 cell viability counter. Differential cell counts were determined by flow cytometry using a Guava easyCyte flow cytometer with a minimum of 100,000 cells per sample. Cells from BALF were stained with CD16/32 (Tonbo biosciences 70-0161-u500, 1:10) to block nonspecific binding and then cells were stained using a master mix of CD45.1 (Biolegend Cat# 110728, 1:1000, San Diego, CA, USA), F4/80 (Biolegend Cat# 123110, 1:500, San Diego, CA, USA), Ly6 B.2 (Novus Biological Cat# NBP2-13077, 1:250, Littleton, CO, USA), CD4 (Invitrogen Cat# 25-0041-82, 1:500, Carlsbad, CA, USA), and CD8 (Invitrogen Cat# 17-0081-82, 1:500, Carlsbad, CA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!