Larval filets of wildtype or Ret mutant animals carrying a C4da neuron specific marker (ppk-Gal4 > CD4-tdGFP) were prepared and immunostained as described above with rabbit anti-phospho-Ret (1:50, Cell Signaling Technology) or guinea pig anti-Ret (1:1000) antibodies. We quantified the Ret antibody signal of C4da neuron somata in confocal image stacks and calculated signal over background ratios (ΔF/F) by subtracting averaged background signal flanking the C4da soma region (F). Statistical significance for the two genotypes was calculated using a two-tailed t-test.
Rabbit anti phospho ret
Manufactured by Cell Signaling Technology
Rabbit anti-phospho-Ret is a primary antibody that specifically recognizes the phosphorylated form of the Ret receptor tyrosine kinase. It is designed for use in various immunoassay applications, such as Western blotting, to detect and analyze the activation status of Ret in biological samples.
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2 protocols using rabbit anti phospho ret
1
Quantifying Ret Signaling in C4da Neurons
2
Drosophila Larval Filet Preparation and Staining
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Larval filet preparation and staining was essentially performed as described (Han et al., 2012 (link)). The following antibodies were used: rabbit anti-phospho-Ret (1:50, Cell Signaling Technology, Danvers, MA), mouse anti-GFP (1:100, Roche Diagnostics, Mannheim, Germany), mouse anti-mys (1:200, Developmental Studies Hybridoma Bank, (DSHB), Iowa City, IO), mouse anti-mew (1:50, DSHB). Secondary DyLight or Alexa conjugated donkey antibodies were from Jackson ImmunoResearch (Westgrove, PA) and were used at 1:400–1:1000.
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