Anti mouse cd103 clone 2e7

Manufactured by BioLegend

Anti-mouse CD103 (clone 2E7) is a lab equipment product. It is a monoclonal antibody that specifically binds to the mouse CD103 protein.

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Lab products found in correlation

Mouse anti guinea pig cd8 clone mca752f, by Bio-Rad (2 mentions) Mouse anti guinea pig cd4 clone mca749pe, by Bio-Rad (2 mentions) Anti mouse crtam clone 11 5, by BioLegend (2 mentions) Hamster anti mouse pd 1 clone j43, by BD (2 mentions) Anti mouse cd69 clone h1.2f3, by BD (2 mentions) Anti mouse cxcr3 clone cxcr3 173, by BioLegend (2 mentions) Anti mouse human cd44 clone im7, by BioLegend (2 mentions)

2 protocols using anti mouse cd103 clone 2e7

Multiparametric Flow Cytometry for Immune Profiling

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Vaginal mucosa cells and splenocytes were analyzed by flow cytometry using the following antibodies: mouse anti-guinea pig CD8 (clone MCA752F, Bio-Rad Laboratories, Hercules, CA), mouse anti-guinea pig CD4 (clone MCA749PE, Bio-Rad Laboratories), anti-mouse CRTAM (clone 11–5, Biolegend, San Diego, CA), hamster anti-mouse PD-1 clone J43, BD Biosciences, San Jose, CA), anti-mouse/human CD44 (clone IM7, Biolegend), anti-mouse CD69 (clone H1.2F3, BD Biosciences, San Jose, CA), anti-mouse CXCR3 (clone CXCR3-173, Biolegend) and anti-mouse CD103 (clone 2E7, Biolegend). For surface staining, mAbs against various cell markers were added to a total of 1X10⁶ cells in phosphate-buffered saline containing 1% FBS and 0.1% sodium azide (fluorescence-activated cell sorter [FACS] buffer) and left for 45 minutes at 4°C. At the end of the incubation period, the cells were washed twice with FACS buffer. A total of 100,000 events were acquired by the LSRII (Becton Dickinson, Mountain View, CA), followed by analysis using FlowJo software (TreeStar, Ashland, OR).

Quadiri A., Prakash S., Dhanushkodi N.R., Singer M., Zayou L., Shaik A.M., Sun M., Suzer B., Lau L., Chilukurri A., Vahed H., Schaefer H, & BenMohamed L. (2023). Therapeutic Prime/Pull Vaccination of HSV-2 Infected Guinea Pigs with the Ribonucleotide Reductase 2 (RR2) Protein and CXCL11 Chemokine Boosts Antiviral Local Tissue-Resident and Effector Memory CD4+ and CD8+ T Cells and Protects Against Recurrent Genital Herpes. bioRxiv.

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Comprehensive Guinea Pig Immune Cell Analysis

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Vaginal mucosa cells and splenocytes were analyzed by flow cytometry using the following antibodies: mouse anti-guinea pig CD8 (clone MCA752F, Bio-Rad Laboratories, Hercules, CA), mouse anti-guinea pig CD4 (clone MCA749PE, Bio-Rad Laboratories), anti-mouse CRTAM (clone 11-5, Biolegend, San Diego, CA), hamster antimouse PD-1 clone J43, BD Biosciences, San Jose, CA), anti-mouse/human CD44 (clone IM7, Biolegend), antimouse CD69 (clone H1.2F3, BD Biosciences, San Jose, CA), anti-mouse CXCR3 (clone CXCR3-173, Biolegend) and anti-mouse CD103 (clone 2E7, Biolegend). For surface staining, mAbs against various cell markers were added to a total of 1X10 6 cells in phosphate-buffered saline containing 1% FBS and 0.1% sodium azide (fluorescence-activated cell sorter [FACS] buffer) and left for 45 minutes at 4°C. At the end of the incubation period, the cells were washed twice with FACS buffer. A total of 100,000 events were acquired by the LSRII (Becton Dickinson, Mountain View, CA), followed by analysis using FlowJo software (TreeStar, Ashland, OR).

Quadiri A., Prakash S., Dhanushkodi N.R., Singer M., Zayou L., Shaik A.M., Sun M., Suzer B., Lau L.S.L., Chilukurri A., Vahed H., Schaefer H, & BenMohamed L. (2024). Therapeutic prime/pull vaccination of HSV-2-infected guinea pigs with the ribonucleotide reductase 2 (RR2) protein and CXCL11 chemokine boosts antiviral local tissue-resident and effector memory CD4⁺ and CD8⁺ T cells and protects against recurrent genital herpes. Journal of virology, 98(5).

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