Tissues or cells were lysed with RIPA buffer. Protein content in lysates was measured using bicinchoninic acid and equal amounts of proteins (20 μg) were separated using SDS-PAGE with β-Actin as a loading control. Separated proteins were transferred to PVDF membranes (Millipore, USA) and subsequently blocked with 5% nonfat dry milk. The membranes were incubated overnight at 4 °C with primary antibodies. The following primary antibodies were used: CPNE1 (1:1000, abcam, ab155675), ATROGIN1 (1:1000, Abcam, ab168372), MuRF1 (1:1000, Abcam, ab183094), MyoG (1:1000, Abcam, ab124800), MyoD (1:1000, Abcam, ab133627), GLB1 (1:1000, Abcam, ab203749), p-eIF2α (1:1000, Cell Signaling Technologies, #9721), eIF2α (1:1000, Abcam, ab169528), p-PERK (1:1000, Cell Signaling Technologies, #3179), PERK (1:1000, Abcam, ab229912), ATF4 (1:1000, Abcam, ab31390) and β-Actin (1:1000, Abcam, ab8226). Protein gray values were measured using Image J software.
Ab169528
Manufactured by Cell Signaling Technology
Ab169528 is a primary antibody developed by Cell Signaling Technology. It is used for the detection of target proteins in various laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Ab124800, by Abcam (1 mentions)
Ab183094, by Abcam (1 mentions)
Ab31390, by Abcam (1 mentions)
Ab133627, by Abcam (1 mentions)
Ab168372, by Abcam (1 mentions)
Ab155675, by Abcam (1 mentions)
Ab229912, by Abcam (1 mentions)
P perk, by Cell Signaling Technology (1 mentions)
P eif2α, by Cell Signaling Technology (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Ab109414, by Abcam (1 mentions)
Ab21685, by Abcam (1 mentions)
Parkin, by Cell Signaling Technology (1 mentions)
Gapdh, by Thermo Fisher Scientific (1 mentions)
Image lab software, by Bio-Rad (1 mentions)
Grp78, by Abcam (1 mentions)
2 protocols using ab169528
1
Protein Expression Analysis in Tissue Lysates
2
Western Blot Analysis of ER Stress Markers
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Protein concentrations were determined using the BCA protein assay kit according to the manufacturer's protocol. Western blotting (WB) was conducted as previously described. Primary antibodies were listed as follows: CSE, Abcam, ab96755; CHOP, Cell Signaling Technology, 2895S; GRP78, Abcam, ab21685; eIF2a, Abcam, ab169528; ATF-6, Cell Signaling Technology, #65880; Parkin, Cell Signaling Technology, #2132; ATG7, Cell Signaling Technology, #2631 ; Nix, Abcam, ab109414; LC-3, Abcam, ab192890; GAPDH, ThermoFisher, MA5-15738-D680. The grayscale value of the bands was quanti ed using Image lab software (BIO-RAD, USA).
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