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Dye conjugated secondary antibodies

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Dye-conjugated secondary antibodies are laboratory reagents used in various immunoassay techniques. They are designed to bind to the primary antibody and provide a detectable signal, typically through a fluorescent dye or enzyme label, to facilitate the visualization and quantification of target molecules in biological samples.

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Lab products found in correlation

H3k27me3, by GeneTex (2 mentions) H2ak119ub, by Cell Signaling Technology (2 mentions) Hnrnpk, by Proteintech (1 mentions) H3k27me3, by Active Motif (1 mentions) Hnrnpk, by Fortis Life Sciences (1 mentions) H3k27me3, by Cell Signaling Technology (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions)

2 protocols using dye conjugated secondary antibodies

1

Chromatin Immunoprecipitation and Microscopy Techniques

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The following primary antibodies were used for ChIP-seq: H3K27me3 (GeneTex, GTX60892), H2AK119ub (Cell Signaling, CST8240); for IF: H3K27me3 (GeneTex, GTX60892), H3K27me3 (Active Motif, AM39535), H2AK119ub (Cell Signaling, CST8240), EZH2 (Cell Signaling, CST3147), HNRNPK (Proteintech, 11426-1-AP), HNRNPK (Bethyl Laboratories, A300-674A), HNRNPE1 (Proteintech, 14523-1-AP), HNRNPE2 (GeneTex, GTX114616), HNRNPE3 (GeneTex, GTX118656), CIZ1 (in-house (Sunwoo et al., 2017 )); for Western blot: H3K27me3 (GeneTex, GTX60892), H3K27me3 (Cell Signaling, CST9733), H2AK119ub (Cell Signaling, CST8240), GAPDH (Cell Signaling, CST14C10), HNRNPK (Proteintech, 11426-1-AP). Dye-conjugated secondary antibodies were purchased from Life Technologies.
Colognori D., Sunwoo H., Kriz A., Wang C.Y, & Lee J.T. (2019). Xist deletional analysis reveals a co-dependency between Xist RNA and Polycomb complexes for spreading along the inactive X. Molecular cell, 74(1), 101-117.e10.
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2

Chromatin Immunoprecipitation and Immunofluorescence Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following primary antibodies were used for ChIP-seq and IF: H3K27me3 (GeneTex, GTX60892) and H2AK119ub (Cell Signaling, CST8240). Dye-conjugated secondary antibodies were purchased from Life Technologies.
Colognori D., Sunwoo H., Wang D., Wang C.Y, & Lee J.T. (2020). Xist Repeats A and B account for two distinct phases of X-inactivation establishment. Developmental cell, 54(1), 21-32.e5.
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