Co2 tri gas incubator

Manufactured by Astec

Sourced in Japan

The CO2/tri-gas incubator is a laboratory equipment designed to provide a controlled environment for cell culture and research applications. It maintains a stable temperature, humidity, and gas composition, with the ability to regulate CO2 and other gas levels as required.

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Lab products found in correlation

Type 1 collagen coated dishes, by BD (2 mentions) Mlo y4, by Kerafast (1 mentions)

2 protocols using co2 tri gas incubator

Hypoxia-Induced Osteocyte Cell Culture

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MLO-Y4 (kerafast, Boston, USA) murine cultured osteocytic cells, which have been used previously, were cultured 16 (link). The cells were plated on type I collagen-coated dishes (BD Biosciences, Bedford, USA) and cultured in α-minimal essential medium (α-MEM) supplemented with 2.5% (v/v) FBS, 2.5% (v/v) FCS, streptomycin (100 μg/ml) and penicillin(100units/ml) 17 (link). Then for the hypoxia experiments, the cells were incubated for 24h in a CO₂/tri-gas incubator (Astec, Fukuoka, Japan) set at a mixture of 5% (v/v) CO₂ and 1% (v/v) O₂ balanced with N₂5 (link).

Ueda S., Shimasaki M., Ichiseki T., Hirata H., kawahara N, & Ueda Y. (2020). Mitochondrial Transcription Factor A added to Osteocytes in a Stressed Environment has a Cytoprotective Effect. International Journal of Medical Sciences, 17(9), 1293-1299.

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Hypoxic Osteocytic Cell Culture

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MLO-Y4 murine cultured osteocytic cells, which were used previously, were cultured [20] (link). The cells were plated on type I collagen-coated dishes (BD Biosciences, Bedford, MA, USA) and cultured in α-minimal essential medium (α-MEM) supplemented with 2.5% (v/v) FBS, 2.5% (v/v) FCS, streptomycin (100 µg/mL) and penicillin (100 units/mL). Then, for the hypoxia experiments, the cells were incubated for 24 h in a CO 2 /tri-gas incubator (Astec, Fukuoka, Japan) set to a mixture of 5% (v/v) CO 2 and 1% (v/v) O 2 balanced with N 2 (hypoxia).

Ueda S., Ichiseki T., Shimasaki M., Hirata H., Kawahara N., & Ueda Y. (2021). Glucocorticoid-Induced Osteocytic Cell Death in a Hypoxic Environment Is Associated with Necroptosis. BioChem, 1(2), 98-106.

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