P06g 1.5 20 f

Zebrafish were anesthetized in fish water containing tricaine and then and mounted onto optical bottom plates (Mat Tek Corporation, P06G-1.5-20-F) in 1% low melting point agarose (Invitrogen, 16520-100) as previously described (Takaki et al., 2013 (link)). Microscopy was performed using a Nikon A1 confocal laser scanning confocal microscopy with a 20x Plan Apo 0.75 NA objective and a Galvano scanner, acquiring 30-80 μm z-stacks with 2-3 μm z-step intervals. Timelapse microscopy was performed at physiological temperature using a heat chamber set to 28°C (Okolab) with an acquisition interval of 2.5-3 min. For multi-day timelapse imaging (Figures 2A and S2), zebrafish larvae were carefully removed using jeweler's forceps and returned to their standard housing (see husbandry) for imaging at later timepoints.

Zebrafish were anesthetized in fish water containing tricaine and then and mounted onto optical bottom plates (MatTek Corporation, P06G-1.5-20-F) in 1% low melting point agarose (Invitrogen, 16520–100) as previously described [5 (link)]. Microscopy was performed using a Nikon A1 confocal laser scanning confocal microscopy with a 20x Plan Apo 0.75 NA objective and a Galvano scanner, acquiring 30–80 μm z-stacks with 2–3 μm z-step intervals. Timelapse microscopy was performed at physiological temperature using a heat chamber set to 28°C (Okolab) with an acquisition interval of 3 minutes.